| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86052-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71573 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54937-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99564-50ul | |||
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP1-86052-img0009.jpg "Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP1-86052-img0007.jpg)
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052] - Staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP1-86052-img0008.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-86052_rabbit-polyclonal-bckdk-antibody-24420259321565.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0007.jpg "Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP2-15553-img0003.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0002.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (NBP2-15553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15553_rabbit-polyclonal-bckdk-antibody-910202420104442.jpg)
![Western Blot: BCKDK Antibody (11C9) [NBP1-47664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.](http://images.novusbio.com/fullsize/BCKDK-Antibody-11C9-Western-Blot-NBP1-47664-img0001.jpg "Western Blot: BCKDK Antibody (11C9) [NBP1-47664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.")
![Immunocytochemistry/Immunofluorescence: BCKDK Antibody (11C9) [NBP1-47664] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.](http://images.novusbio.com/fullsize/BCKDK-Antibody-11C9-Immunocytochemistry-Immunofluorescence-NBP1-47664-img0004.jpg)
![Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCKDK-Antibody-11C9-Immunohistochemistry-Paraffin-NBP1-47664-img0003.jpg)
![Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human endometrium tissue using anti-BCKDK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCKDK-Antibody-11C9-Immunohistochemistry-Paraffin-NBP1-47664-img0002.jpg)
![Western Blot: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.](http://images.novusbio.com/fullsize/BCKDK-Antibody-OTI11C9-Azide-and-BSA-Free-Western-Blot-NBP2-71573-img0001.jpg "Western Blot: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.")
![Immunocytochemistry/Immunofluorescence: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.](http://images.novusbio.com/fullsize/BCKDK-Antibody-OTI11C9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71573-img0002.jpg)
![Immunohistochemistry: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - Staining of paraffin-embedded Human endometrium tissue using anti-BCKDK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCKDK-Antibody-OTI11C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71573-img0003.jpg)
![Immunohistochemistry: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - Staining of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCKDK-Antibody-OTI11C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71573-img0004.jpg)
![BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.BCKDK stained by BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48724_rabbit-bckdk-mab-hl2710-immunohistochemistry-paraffin-1411202518393118.jpg "BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.BCKDK stained by BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48724_rabbit-bckdk-mab-hl2710-western-blot-1411202518314516.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48724_rabbit-bckdk-mab-hl2710-western-blot-1411202518381723.jpg)
![Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48724_rabbit-bckdk-mab-hl2710-western-blot-1411202518335911.jpg)
![Western Blot: BCKDK Antibody (5J3L3) [NBP3-33228] - Western blot analysis of various lysates using BCKDK Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33228_rabbit-bckdk-mab-5j3l3-912202415175299.jpg "Western Blot: BCKDK Antibody (5J3L3) [NBP3-33228] - Western blot analysis of various lysates using BCKDK Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: BCKDK Antibody (S01-3G4) [NBP3-19836] - Western blot detection of BCKDK in Hela, A549, HL-60 using NBP3-19836 (1:1000 diluted)](http://images.novusbio.com/fullsize/BCKDK-Antibody-S01-3G4-Western-Blot-NBP3-19836-img0001.jpg "Western Blot: BCKDK Antibody (S01-3G4) [NBP3-19836] - Western blot detection of BCKDK in Hela, A549, HL-60 using NBP3-19836 (1:1000 diluted)")
![Immunocytochemistry/Immunofluorescence: BCKDK Antibody [NBP2-54937] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54937-img0001.jpg "Immunocytochemistry/Immunofluorescence: BCKDK Antibody [NBP2-54937] - Staining of human cell line U-2 OS shows localization to mitochondria.")
![Western Blot: BCKDK Antibody [NBP1-57574] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP1-57574-img0002.jpg "Western Blot: BCKDK Antibody [NBP1-57574] - Human Brain lysate, concentration 0.2-1 ug/ml.")
![Western Blot: BCKDK Overexpression Lysate (Adult Normal) [NBL1-07945] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BCKDK.](http://images.novusbio.com/fullsize/BCKDK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07945-img0002.jpg "Western Blot: BCKDK Overexpression Lysate (Adult Normal) [NBL1-07945] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BCKDK.")
![Western Blot: BCKDK Antibody [NBP2-99564] - Anti-BCKDK rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-99564-img0002.jpg "Western Blot: BCKDK Antibody [NBP2-99564] - Anti-BCKDK rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa")