HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6015-SP | |||
HAF016). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PDCD6 Polyclonal Antibody (Catalog # AF6015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6015-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94061-0.02ml | |||
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Polyclonal PDCD6 Antibody [NBP2-94061] - Analysis of extracts from wild type (WT) and PDCD6 knockout (KO) 293T(KO) cells, using PDCD6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-94061_rabbit-polyclonal-pdcd6-antibody-knockout-validated-2382023104054.jpg)
![Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32728_mouse-pdcd6-mab-a6a7-r-292024111480.jpg "Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Flow cytometric analysis of HeLa cells labeling PDCD6.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32728, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32728_mouse-pdcd6-mab-a6a7-r-392024884677.jpg)
![Western blot analysis on different lysates with NBP3-32728 at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32728 at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32728_-western-blot-639204590076785133.jpg)
![Western Blot: PDCD6 Overexpression Lysate (Adult Normal) [NBL1-14209] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDCD6.](http://images.novusbio.com/fullsize/PDCD6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14209-img0002.jpg "Western Blot: PDCD6 Overexpression Lysate (Adult Normal) [NBL1-14209] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDCD6.")
![SDS-Page: Recombinant Human PDCD6 Protein [NBP1-51048] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-PDCD6-Protein-SDS-Page-NBP1-51048-img0001.jpg "SDS-Page: Recombinant Human PDCD6 Protein [NBP1-51048] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![ELISA: Human PDCD6 ELISA Kit (Colorimetric) [NBP3-39456] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39456_human-pdcd6-elisa-kit-colorimetric-2110202415371365.png "ELISA: Human PDCD6 ELISA Kit (Colorimetric) [NBP3-39456] - Standard Curve Reference")
![ELISA: Human PDCD6 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39457] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39457_human-pdcd6-ready-to-use-elisa-kit-colorimetric-211020241529177.png "ELISA: Human PDCD6 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39457] - Standard Curve Reference")
