Conjugated Primary Antibodies: Find Your Antibody and Conjugate of Interest

Related Links

Indirect vs Direct Detection

Janelia Fluor® Dyes

Carrier-free Antibodies

Spectra Viewer

Flow Cytometry Panel Builder

Custom Conjugation Services

Conjugated Antibodies Poster

Antibody Labeling Methods

Antibody Conjugation Illustrated Assay

Tandem Dye Conjugated Antibodies FAQs

Application Handbooks and Guides

5 Pillars of Antibody Validation

Find your antibody and conjugate of interest using the search tool below.


Browse all conjugated primary antibodies


Conjugated Primary Antibodies

A conjugated antibody (also known as a tagged or labeled antibody) is a monoclonal or polyclonal antibody that is coupled to a probe allowing for direct detection of a target antigen. Novus Biologicals offers more than 85,000 primary antibodies directly conjugated to 20 different fluorescent or enzymatic labels including a wide range of Alexa Fluor® dyes and FITC conjugated antibodies. Novus conjugated primaries will help you generate clean, reproducible results in a variety of applications such as Flow Cytometry, ICC, IHC, and ELISA.


Conjugated Antibodies for Research Areas


Popular Conjugates for Primary Antibodies

Emission Color Conjugate Excitation Max (nm) Emission Max (nm)
    HRP    
    Biotin    
     Blue DyLight 405 400 420
     Blue Alexa Fluor® 405 401 421
     Blue DyLight 350 353 432
     Blue Alexa Fluor® 350 346 442
     Green DyLight 488 493 518
     Green FITC 498 519
     Green Alexa Fluor® 488 490 525
     Yellow Alexa Fluor® 532 532 554
     Yellow Janelia Fluor® 549 549 571
     Yellow DyLight 550 562 576
     Yellow PE 565 578
     Orange Alexa Fluor® 594 590 617
     Orange DyLight 594 593 618
     Orange PE/Atto594* 498,544,566 632
     Red APC 650 660
     Red Janelia Fluor® 646 646 664
     Red Alexa Fluor® 647 653 669
     Red DyLight 650 652 672
     Red PerCP 482/678 678
     Red PE/Cy5.5* 498,544,566 700
     Near IR DyLight 680 692 712
     Near IR Alexa Fluor® 700 696 720
     Near IR Alexa Fluor® 750 749 775
     Near IR DyLight 755 752 778
     Near IR PE/Cy7* 489,544,566 782
     Near IR APC/Cy7 652 790
*These dyes have three maximum excitation wavelengths. All three can be used; optimal excitation will depend on application.

Conjugated Antibody Support

Novus Spectra Viewer
Visualize the spectral profile of your fluorescent probe and identify compatible fluorophore conjugates for your multicolor imaging or flow experiment.

Direct vs Indirect Detection Methods
Learn more about the benefits of direct versus indirect detection methods in fluorescence-based immunodetection.

Flow Cytometry Handbook
Our step-by-step guide provides information on the basics of flow cytometry and troubleshooting techniques, as well as a list of available conjugates across multiple wavelengths to help maximize data output in your experiments.

Custom Antibody Conjugation Services
Can’t find the conjugated primary antibody you’re looking for? At Novus, we provide custom conjugation services for many of our available primary antibodies to meet your research needs.

 

Example Data Using Conjugated Primary Antibodies

NeuN was detected in mouse cortical cells by ICC/IF using Alexa Fluor® 700 conjugated RBFOX3/NeuN antibody (1B7)NeuN was detected in mouse cortical cells ICC/IF using Alexa Fluor® 700 conjugated RBFOX3/NeuN antibody (1B7) [NBP1-92693AF700] Image from a verified customer review.

VeroB4 cells were stained with Alexa Fluor® 594 conjugated Flavivirus group antigen antibody at 1:400. The left panel are uninfected cells and the right panel are cells infected with Yellow Fever 17D Venus Virus.

VeroB4 cells were stained with Alexa Fluor® 594 conjugated Flavivirus group antigen antibody (D1-4G2-4-15 (4G2)) [NBP2-52666AF594] at 1:400 for Flow Cytometry analysis. The left panel are uninfected cells and the right panel are cells infected with Yellow Fever 17D Venus Virus.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.