MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="HeLa cells were stained with Mouse Anti-Human Insulysin/IDE Monoclonal Antibody (Catalog # MAB2496, filled histogram) or isotype control antibody (Catalog #
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="HeLa cells were stained with Mouse Anti-Human Insulysin/IDE Monoclonal Antibody (Catalog # MAB2496, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
![Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Wild-type (WT) and Insulysin/IDE knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT143-Western-Blot-NBP3-13560-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Wild-type (WT) and Insulysin/IDE knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Insulysin/IDE antibody [GT143] detects Insulysin/IDE protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT143-Immunohistochemistry-Paraffin-NBP3-13560-img0003.jpg)
![Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT143-Western-Blot-NBP3-13560-img0001.jpg)
![Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT143-Western-Blot-NBP3-13560-img0004.jpg)
![Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Insulysin/IDE antibody [GT143] detects Insulysin/IDE protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT143-Immunohistochemistry-Paraffin-NBP3-13560-img0002.jpg)
![Western Blot: Insulysin/IDE Antibody (GT286) [NBP3-13547] - Wild-type (WT) and Insulysin/IDE knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT286] (NBP3-13547) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT286-Western-Blot-NBP3-13547-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Insulysin/IDE Antibody (GT286) [NBP3-13547] - Wild-type (WT) and Insulysin/IDE knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT286] (NBP3-13547) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT286) [NBP3-13547] - Insulysin/IDE antibody [GT286] detects Insulysin/IDE protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Insulysin/IDE antibody [GT286] (NBP3-13547) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT286-Immunohistochemistry-Paraffin-NBP3-13547-img0002.jpg)
![Western Blot: Insulysin/IDE Antibody (GT286) [NBP3-13547] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT286] (NBP3-13547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-GT286-Western-Blot-NBP3-13547-img0003.jpg)
![Western Blot: Insulysin/IDE Antibody (GT286) [NBP3-13547] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT286] (NBP3-13547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13547_mouse-insulysin-ide-mab-gt286-91020242011428.jpg)
![Western Blot: Insulysin/IDE Antibody [NB100-68148] - K562 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Western-Blot-NB100-68148-img0009.jpg "Western Blot: Insulysin/IDE Antibody [NB100-68148] - K562 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Insulysin/IDE Antibody [NB100-68148] - Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer (pH 6), AP-staining.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Immunohistochemistry-Paraffin-NB100-68148-img0010.jpg)
![Immunohistochemistry-Paraffin: Insulysin/IDE Antibody [NB100-68148] - Human Lung tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Immunohistochemistry-Paraffin-NB100-68148-img0006.jpg)
![Western Blot: Insulysin/IDE Antibody [NBP3-03392] - Analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using Insulysin/IDE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Western-Blot-NBP3-03392-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Insulysin/IDE Antibody [NBP3-03392] - Analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using Insulysin/IDE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Insulysin/IDE Antibody [NBP3-03392] - Analysis of extracts of various cell lines, using Insulysin/IDE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Western-Blot-NBP3-03392-img0003.jpg)
![Immunohistochemistry: Insulysin/IDE Antibody - BSA Free [Insulysin/IDE] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Insulysin/IDE Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03392_rabbit-polyclonal-insulysin-ide-antibody-52202516283293.jpg)
![Immunocytochemistry/ Immunofluorescence: Insulysin/IDE Antibody - BSA Free [Insulysin/IDE] - Immunofluorescence analysis of A549 cells using [KO Validated] Insulysin/IDE Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03392_rabbit-polyclonal-insulysin-ide-antibody-5220251639677.jpg)
![Immunocytochemistry/Immunofluorescence: Insulysin/IDE Antibody (S04-4E8) [NBP3-19987] - Immunocytochemistry of Insulysin/IDE (green) in hela using NBP3-19987 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19987_rabbit-insulysin-ide-mab-s04-4e8-113202515145425.jpg "Immunocytochemistry/Immunofluorescence: Insulysin/IDE Antibody (S04-4E8) [NBP3-19987] - Immunocytochemistry of Insulysin/IDE (green) in hela using NBP3-19987 at dilution 1/50, and DAPI (blue)")
![Western Blot: Insulysin/IDE Antibody [NBP2-38994] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Western-Blot-NBP2-38994-img0003.jpg "Western Blot: Insulysin/IDE Antibody [NBP2-38994] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Insulysin/IDE Antibody [NBP2-38994] - Staining of human stomach shows membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Insulysin-IDE-Antibody-Immunohistochemistry-Paraffin-NBP2-38994-img0004.jpg)
![ELISA: Human Insulysin/IDE ELISA Kit (Colorimetric) [NBP2-68029] - Samples were spiked with high concentrations of Human Insulysin/IDE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68029_human-insulysin-ide-elisa-kit-colorimetric-132202416173449.jpg "ELISA: Human Insulysin/IDE ELISA Kit (Colorimetric) [NBP2-68029] - Samples were spiked with high concentrations of Human Insulysin/IDE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Insulysin/IDE ELISA Kit (Colorimetric) [NBP2-68029] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68029_human-insulysin-ide-elisa-kit-colorimetric-432024903586.jpg)
![ELISA: Human Insulysin/IDE ELISA Kit (Chemiluminescence) [NBP2-68028] - Samples were spiked with high concentrations of Human Insulysin/IDE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68028_human-insulysin-ide-elisa-kit-chemiluminescence-13220241612132.jpg "ELISA: Human Insulysin/IDE ELISA Kit (Chemiluminescence) [NBP2-68028] - Samples were spiked with high concentrations of Human Insulysin/IDE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Insulysin/IDE ELISA Kit (Chemiluminescence) [NBP2-68028] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68028_human-insulysin-ide-elisa-kit-chemiluminescence-4320249141649.jpg)
