Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97512-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00189-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90208-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06258-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06403-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05939-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00106-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97696-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12701-50ul | |||
![Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP2-97512-img0005.jpg "Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa")
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-97512-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-97512-img0003.jpg)
![Immunoprecipitation: ATP5H Antibody [NBP2-97512] - ATP5H was immunoprecipitated using: Lane A: 0.5 mg PC-3 Whole Cell Lysate. 4 ul anti-ATP5H rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ATP5H rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunoprecipitation-NBP2-97512-img0006.jpg)
![Immunohistochemistry-Paraffin: CNPY2 Antibody [NBP3-00189] - Immunochemical staining of human CNPY2 in human pancreas with NBP3-00189 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00189_rabbit-polyclonal-cnpy2-antibody-235202511545685.png "Immunohistochemistry-Paraffin: CNPY2 Antibody [NBP3-00189] - Immunochemical staining of human CNPY2 in human pancreas with NBP3-00189 (1:5000, formalin-fixed paraffin embedded sections).")
![Western Blot: CNPY2 Antibody [NBP3-00189] - NBP3-00189 at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:17 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00189_rabbit-polyclonal-cnpy2-antibody-23520251664758.png)
![Immunohistochemistry-Paraffin: CNPY2 Antibody [NBP3-00189] - Immunochemical staining of human CNPY2 in human placenta with NBP3-00189 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00189_rabbit-polyclonal-cnpy2-antibody-235202514492354.png)
![Immunoprecipitation: CNPY2 Antibody [NBP3-00189] - CNPY2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate 2 uL NBP3-00189 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00189, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size: kDa](http://images.novusbio.com/fullsize/nbp3-00189_rabbit-polyclonal-cnpy2-antibody-235202521525010.png)
![Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Flow-Cytometry-NBP2-90208-img0003.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t")
![<b>Biological Strategies Validation. </b>Western Blot: ERp72 Antibody (020) [NBP2-90208] - Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Western-Blot-NBP2-90208-img0006.jpg)
![Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208] - PDIA4 was immunoprecipitated using: Lane A :0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Immunoprecipitation-NBP2-90208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] - Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90208 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90208_rabbit-monoclonal-erp72-antibody-020-235202511545653.png)
![Immunohistochemistry: NARFL Antibody [NBP3-06258] - Immunochemical staining of human NARFL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NARFL-Antibody-Immunohistochemistry-NBP3-06258-img0005.jpg "Immunohistochemistry: NARFL Antibody [NBP3-06258] - Immunochemical staining of human NARFL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: NARFL Antibody [NBP3-06258] - Anti-NARFL rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:57 kDa](http://images.novusbio.com/fullsize/NARFL-Antibody-Western-Blot-NBP3-06258-img0001.jpg)
![Immunohistochemistry-Paraffin: NARFL Antibody [NBP3-06258] - Immunochemical staining of human NARFL in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NARFL-Antibody-Immunohistochemistry-Paraffin-NBP3-06258-img0004.jpg)
![Immunoprecipitation: NARFL Antibody [NBP3-06258] - NARFL was immunoprecipitated using:Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-NARFL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-NARFL rabbit polyclonal antibody, at 1:100 dilution.Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 56 kDaObserved band size :56 kDa](http://images.novusbio.com/fullsize/NARFL-Antibody-Immunoprecipitation-NBP3-06258-img0002.jpg)
![Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - AUP1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AUP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AUP1 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :43 kDa.](http://images.novusbio.com/fullsize/Ancient-ubiquitous-protein-1-Antibody-Western-Blot-NBP3-06403-img0004.jpg "Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - AUP1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AUP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AUP1 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :43 kDa.")
![Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - Immunochemical staining of human AUP1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Ancient-ubiquitous-protein-1-Antibody-Immunohistochemistry-Paraffin-NBP3-06403-img0003.jpg)
![Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - Anti-AUP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: RPMI-8226 Whole Cell Lysate Lane B: NIH/3T3 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa.](http://images.novusbio.com/fullsize/Ancient-ubiquitous-protein-1-Antibody-Western-Blot-NBP3-06403-img0001.jpg)
![Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - Immunochemical staining of human AUP1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Ancient-ubiquitous-protein-1-Antibody-Immunohistochemistry-Paraffin-NBP3-06403-img0002.jpg)
![Western Blot: DNA Polymerase beta Antibody [NBP3-05939] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Western-Blot-NBP3-05939-img0001.jpg "Western Blot: DNA Polymerase beta Antibody [NBP3-05939] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa")
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939] - Staining of human POLB in human testis with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-05939-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939] - Staining of human POLB in human gallbladder with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunohistochemistry-Paraffin-NBP3-05939-img0002.jpg)
![Immunoprecipitation: DNA Polymerase beta Antibody [NBP3-05939] - Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-POLB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-POLB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size :40 kDa](http://images.novusbio.com/fullsize/DNA-Polymerase-beta-Antibody-Immunoprecipitation-NBP3-05939-img0004.jpg)
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Western-Blot-NBP2-97692-img0005.jpg "Western Blot: PCYT1A Antibody [NBP2-97692] - Anti-PCYT1A rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human skin with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0004.jpg)
![Immunohistochemistry-Paraffin: PCYT1A Antibody [NBP2-97692] - Staining of human PCYT1A in human esophagus with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-97692-img0003.jpg)
![Immunoprecipitation: PCYT1A Antibody [NBP2-97692] - PCYT1A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-PCYT1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PCYT1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/PCYT1A-Antibody-Immunoprecipitation-NBP2-97692-img0006.jpg)
![Western Blot: GM2A Antibody [NBP3-00106] - NBP3-00106 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00106_rabbit-polyclonal-gm2a-antibody-235202511444455.png "Western Blot: GM2A Antibody [NBP3-00106] - NBP3-00106 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: GM2A Antibody [NBP3-00106] - GM2A was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL NBP3-00106 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-00106, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)[HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa](http://images.novusbio.com/fullsize/nbp3-00106_rabbit-polyclonal-gm2a-antibody-23520251633506.png)
![Immunohistochemistry-Paraffin: GM2A Antibody [NBP3-00106] - Immunochemical staining of human GM2A in human kidney with NBP3-00106 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00106_rabbit-polyclonal-gm2a-antibody-235202511354148.png)
![Immunohistochemistry-Paraffin: GM2A Antibody [NBP3-00106] - Immunochemical staining of human GM2A in human skin with NBP3-00106 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00106_rabbit-polyclonal-gm2a-antibody-235202521525064.png)
![Western Blot: PGRMC1 Antibody [NBP2-97696] - Anti-PGRMC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 24 kDa](http://images.novusbio.com/fullsize/PGRMC1-Antibody-Western-Blot-NBP2-97696-img0005.jpg "Western Blot: PGRMC1 Antibody [NBP2-97696] - Anti-PGRMC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 24 kDa")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP2-97696] - Immunochemical staining of human PGRMC1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP2-97696-img0004.jpg)
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP2-97696] - Immunochemical staining of human PGRMC1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP2-97696-img0003.jpg)
![Immunoprecipitation: PGRMC1 Antibody [NBP2-97696] - PGRMC1 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate 4 ul anti-PGRMC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PGRMC1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/PGRMC1-Antibody-Immunoprecipitation-NBP2-97696-img0006.jpg)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Western Blot: Ndufs1 Antibody [NBP3-12701] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:79 kDaObserved band size:72 kDa](http://images.novusbio.com/fullsize/Ndufs1-Antibody-Western-Blot-NBP3-12701-img0001.jpg "Western Blot: Ndufs1 Antibody [NBP3-12701] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:79 kDaObserved band size:72 kDa")
![Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP3-12701] - Staining of human NDUFS1 in human testis with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Ndufs1-Antibody-Immunohistochemistry-Paraffin-NBP3-12701-img0003.jpg)
![Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP3-12701] - Staining of human NDUFS1 in human liver with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Ndufs1-Antibody-Immunohistochemistry-Paraffin-NBP3-12701-img0002.jpg)
![Immunoprecipitation: Ndufs1 Antibody [NBP3-12701] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NDUFS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 79 kDaObserved band size :72 kDa](http://images.novusbio.com/fullsize/Ndufs1-Antibody-Immunoprecipitation-NBP3-12701-img0004.jpg)