Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-74975 | |||
| Biotin | NBP1-74976 | |||
| DyLight 488 | NBP1-74980 | |||
| DyLight 550 | NBP1-75816 | |||
| DyLight 594 | NBP1-75817 | |||
| DyLight 633 | NBP1-75818 | |||
| FITC | NBP1-74977 | |||
| HRP | NBP1-74978 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0230A488-100 | |||
| Alexa Fluor 546 | DDX0230A546-100 | |||
| Alexa Fluor 647 | DDX0230A647-100 | |||
| Biotin | DDX0230B-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19249-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97472-0.2mg | |||
| BSA Free | NBP1-97472-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06040-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97553-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97907-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12616-50ul | |||
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review. Analysis using the DyLight-550 conjugated antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0003.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review. Analysis using the DyLight-550 conjugated antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - MCF7 human breast cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG Fc Secondary Antibody [NBP1-74974] - SKBR3 human breast cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-fragment-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-74974-img0002.jpg)
![Western Blot: PILR-alpha Antibody (36H2) [DDX0230P-100] - FDFO3 is a cell-surface monomeric glycoprotein](http://images.novusbio.com/fullsize/PILR-alpha-Antibody-36H2-Western-Blot-DDX0230P-100-img0004.jpg "Western Blot: PILR-alpha Antibody (36H2) [DDX0230P-100] - FDFO3 is a cell-surface monomeric glycoprotein")
![Flow Cytometry: PILR-alpha Antibody (36H2) [DDX0230P-100] - Surface FACS staining of human PBMC](http://images.novusbio.com/fullsize/PILR-alpha-Antibody-36H2-Flow-Cytometry-DDX0230P-100-img0003.jpg)
![Flow Cytometry: PILR-alpha Antibody (36H2) [DDX0230P-100] - An Ig-SF inhibitory receptor](http://images.novusbio.com/fullsize/PILR-alpha-Antibody-36H2-Flow-Cytometry-DDX0230P-100-img0002.jpg)
![Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD74-Antibody-CerCLIP-1-Flow-Cytometry-NBP1-19249-img0001.jpg "Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg "Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612216.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-3102024161227.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612226.jpg)
![Western Blot: HPV18 E7 Antibody (718-15) [NB110-17215] - Papillomavirus 18 E7 in Hela whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/HPV18-E7-Antibody-718-15-Western-Blot-NB110-17215-img0001.jpg "Western Blot: HPV18 E7 Antibody (718-15) [NB110-17215] - Papillomavirus 18 E7 in Hela whole cell lysate. Image from verified customer review.")
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0004.jpg "Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PerCP-CyTM5.5.")
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) APC.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0001.jpg)
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - [NB500-326-Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) FITC.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0002.jpg)
![Flow Cytometry: CD7 Antibody (MEM-186) [NB500-326] - Surface staining of human peripheral blood cells with anti-human CD7 (MEM-186) PE-CyTM7.](http://images.novusbio.com/fullsize/CD7-Antibody-MEM-186-Flow-Cytometry-NB500-326-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592116.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 3. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420594212.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 2. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-81120242059212.jpg)
![Western Blot: MyoD Antibody (5.2F) [NBP3-30815] - Left: Immunohistochemical staining using MyoD antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD protein with MyoD antibody at 2 ug/ml and detected using goat anti-mouse HRP and visualized with Pierce West-Femto substrate.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592184.jpg)
![Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Western-Blot-NBP3-06040-img0001.jpg "Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa")
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0004.jpg)
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human kidney with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0003.jpg)
![Immunoprecipitation: APBA3/Mint-3 Antibody [NBP3-06040] - APBA3 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-APBA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-APBA3 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa Observed band size :72 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunoprecipitation-NBP3-06040-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of APBA3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06040 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06040_rabbit-polyclonal-apba3-mint-3-antibody-235202514492346.png)
![Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-97553-img0003.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP2-97553-img0004.jpg)
![Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553] - Use1/UBE2Z was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Use1/UBE2Z rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Use1/UBE2Z rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunoprecipitation-NBP2-97553-img0001.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody - BSA Free [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human duodenum with NBP2-97553 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-23520251220550.png)
![Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] - Immunofluorescence staining of Use1/UBE2Z in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97553 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97553_rabbit-polyclonal-use1-ube2z-antibody-235202512205520.png)
![Western Blot: MAP7 Antibody [NBP2-97907] - Lane A: A549 Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:84 kDa](http://images.novusbio.com/fullsize/MAP7-Antibody-Western-Blot-NBP2-97907-img0006.jpg "Western Blot: MAP7 Antibody [NBP2-97907] - Lane A: A549 Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:84 kDa")
![Immunohistochemistry-Paraffin: MAP7 Antibody [NBP2-97907] - Immunochemical staining of human MAP7 in human large intestine with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MAP7-Antibody-Immunohistochemistry-Paraffin-NBP2-97907-img0005.jpg)
![Immunohistochemistry-Paraffin: MAP7 Antibody [NBP2-97907] - Immunochemical staining of human MAP7 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MAP7-Antibody-Immunohistochemistry-Paraffin-NBP2-97907-img0004.jpg)
![Immunoprecipitation: MAP7 Antibody [NBP2-97907] - Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MAP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MAP7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size :115 kDa](http://images.novusbio.com/fullsize/MAP7-Antibody-Immunoprecipitation-NBP2-97907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MAP7 Antibody [NBP2-97907] - Immunofluorescence staining of MAP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97907 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97907_rabbit-polyclonal-map7-antibody-23520251129319.png)
![Western Blot: ZNF346 Antibody [NBP3-12616] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLane D: Jurakt Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:38 kDa](http://images.novusbio.com/fullsize/ZNF346-Antibody-Western-Blot-NBP3-12616-img0001.jpg "Western Blot: ZNF346 Antibody [NBP3-12616] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLane D: Jurakt Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:38 kDa")
![Immunohistochemistry-Paraffin: ZNF346 Antibody [NBP3-12616] - Staining of human ZNF346 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ZNF346-Antibody-Immunohistochemistry-Paraffin-NBP3-12616-img0003.jpg)
![Immunohistochemistry-Paraffin: ZNF346 Antibody [NBP3-12616] - Staining of human ZNF346 in human brain with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ZNF346-Antibody-Immunohistochemistry-Paraffin-NBP3-12616-img0002.jpg)
![Immunoprecipitation: ZNF346 Antibody [NBP3-12616] - Lane A:0.5 mg Jurakt Whole Cell Lysate4 uL anti-ZNF346 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZNF346 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size :38 kDa](http://images.novusbio.com/fullsize/ZNF346-Antibody-Immunoprecipitation-NBP3-12616-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ZNF346 Antibody [NBP3-12616] - Staining of ZNF346 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12616 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-12616_rabbit-polyclonal-znf346-antibody-23520251664712.png)