Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75217 | |||
| Pre-adsorbed | NBP1-75217JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74981 | |||
| Pre-adsorbed | NBP1-74982 | |||
| Pre-adsorbed | NBP1-74983 | |||
| Pre-adsorbed | NBP1-74984 | |||
| Pre-adsorbed | NBP1-74985 | |||
| Pre-adsorbed | NBP1-75827 | |||
| Pre-adsorbed | NBP1-74981-0.5mg | |||
| Pre-adsorbed | NBP1-74981JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75017 | |||
| Pre-adsorbed | NBP1-75018 | |||
| Pre-adsorbed | NBP1-75019 | |||
| Pre-adsorbed | NBP1-75020 | |||
| Pre-adsorbed | NBP1-75021 | |||
| Pre-adsorbed | NBP1-75892 | |||
| Pre-adsorbed | NBP1-75017-0.5mg | |||
| Pre-adsorbed | NBP1-75017JF646 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74995 | |||
| Pre-adsorbed | NBP1-74996 | |||
| Pre-adsorbed | NBP1-74997 | |||
| Pre-adsorbed | NBP1-74999 | |||
| Pre-adsorbed | NBP1-74995-0.5mg | |||
| Pre-adsorbed | NBP1-74995JF646 | |||
![Western Blot: PKC alpha Antibody (2F11) [H00005578-M01] - PRKCA monoclonal antibody (M01), clone 2F11 Analysis of PRKCA expression in HeLa.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Western-Blot-H00005578-M01-img0011.jpg "Western Blot: PKC alpha Antibody (2F11) [H00005578-M01] - PRKCA monoclonal antibody (M01), clone 2F11 Analysis of PRKCA expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00005578-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Immunohistochemistry-Paraffin-H00005578-M01-img0009.jpg)
![Proximity Ligation Assay: PKC alpha Antibody (2F11) [H00005578-M01] - Analysis of protein-protein interactions between FAS and PRKCA. HeLa cells were stained with anti-FAS rabbit purified polyclonal 1:1200 and anti-PRKCA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-Proximity-Ligation-Assay-H00005578-M01-img0005.jpg)
![ELISA: PKC alpha Antibody (2F11) [H00005578-M01] - Detection limit for recombinant GST tagged PRKCA is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-2F11-ELISA-H00005578-M01-img0012.jpg)
![Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection against CHD11-His tag (61.49 KDa).](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-Western-Blot-H00001009-M05-100ug-img0007.jpg "Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection against CHD11-His tag (61.49 KDa).")
![Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-Immunocytochemistry-Immunofluorescence-H00001009-M05-100ug-img0004.jpg)
![Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in MCF-7.](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-Western-Blot-H00001009-M05-100ug-img0005.jpg)
![Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in human kidney.](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-Western-Blot-H00001009-M05-100ug-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-Immunocytochemistry-Immunofluorescence-H00001009-M05-100ug-img0001.jpg)
![ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-ELISA-H00001009-M05-100ug-img0002.jpg)
![ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cadherin-11-Antibody-3C8-ELISA-H00001009-M05-100ug-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NUDT21 Antibody (3F8) [H00011051-M12] - Analysis of monoclonal antibody to NUDT21 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NUDT21-Antibody-3F8-Immunocytochemistry-Immunofluorescence-H00011051-M12-img0003.jpg "Immunocytochemistry/Immunofluorescence: NUDT21 Antibody (3F8) [H00011051-M12] - Analysis of monoclonal antibody to NUDT21 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: NUDT21 Antibody (3F8) [H00011051-M12] - Analysis of monoclonal antibody to NUDT21 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/NUDT21-Antibody-3F8-Immunohistochemistry-Paraffin-H00011051-M12-img0004.jpg)
![ELISA: NUDT21 Antibody (3F8) [H00011051-M12] - Detection limit for recombinant GST tagged NUDT21 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NUDT21-Antibody-3F8-ELISA-H00011051-M12-img0002.jpg)
![Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of GSN expression in transfected 293T cell line by GSN monoclonal antibody (M01), clone 3G5.Lane 1: GSN transfected lysate(85.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-3G5-Western-Blot-H00002934-M01-img0009.jpg "Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of GSN expression in transfected 293T cell line by GSN monoclonal antibody (M01), clone 3G5.Lane 1: GSN transfected lysate(85.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of monoclonal antibody to GSN on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-3G5-Immunohistochemistry-Paraffin-H00002934-M01-img0005.jpg)
![Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - GSN monoclonal antibody (M01), clone 3G5 Analysis of GSN expression in HeLa.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-3G5-Western-Blot-H00002934-M01-img0008.jpg)
![Immunoprecipitation: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of GSN transfected lysate using anti-GSN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSN MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-3G5-Immunoprecipitation-H00002934-M01-img0006.jpg)
![Sandwich ELISA: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Detection limit for recombinant GST tagged GSN is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-3G5-Sandwich-ELISA-H00002934-M01-img0007.jpg)
![Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Western Blot Analysis of Human HeLa cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).](http://images.novusbio.com/fullsize/Spectrin-alpha-1-Antibody-SPTA1-1832-Azide-and-BSA-Free-Western-Blot-NBP2-79906-img0005.jpg "Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Western Blot Analysis of Human HeLa cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).")
![Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Formalin-fixed, paraffin-embedded human Tonsil stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).](http://images.novusbio.com/fullsize/Spectrin-alpha-1-Antibody-SPTA1-1832-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79906-img0003.jpg)
![Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Western Blot Analysis of Human K562 cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).](http://images.novusbio.com/fullsize/Spectrin-alpha-1-Antibody-SPTA1-1832-Azide-and-BSA-Free-Western-Blot-NBP2-79906-img0004.jpg)
![Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).](http://images.novusbio.com/fullsize/Spectrin-alpha-1-Antibody-SPTA1-1832-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79906-img0002.jpg)
![Protein Array: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin alpha 1 Antibody (SPTA1/1832) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Spectrin-alpha-1-Antibody-SPTA1-1832-Azide-and-BSA-Free-Protein-Array-NBP2-79906-img0001.jpg)
![N/A Indoleamine 2,3-dioxygenase/IDO [Biotin]](http://images.novusbio.com/fullsize2/dy6030b-05_human-indoleamine-2-3-dioxygenase-ido-duoset-elisa-5-plate-2842022141620.jpg "N/A Indoleamine 2,3-dioxygenase/IDO [Biotin]")
![Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) - Azide and BSA Free [NBP2-80590] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Bat-Immunoglobulin-Antibody-BT1-4F10-Azide-and-BSA-Free-Western-Blot-NBP2-80590-img0001.jpg "Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) - Azide and BSA Free [NBP2-80590] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized. Image from the standard format of this antibody.")