Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 549
• Format: Pre-adsorbed

Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

• Immunogen: Purified Mouse IgG + IgM
• Specificity: Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and heavy (mu) chains on mouse IgM and light chains on all mouse immunoglobulins. Cross Adsorbed: This antibody has been pre-adsorbed against human IgG and serum proteins to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgG serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and heavy (mu) chains on mouse IgM and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.