Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB71442-SP | |||
| Carrier Free | FAB71442V-100UG | |||
| Carrier Free | FAB71442T-100UG | |||
| Carrier Free | FAB71442G-100UG | |||
| Carrier Free | FAB71442S-100UG | |||
| Carrier Free | FAB71442U-100UG | |||
| Carrier Free | FAB71442R-100UG | |||
| Carrier Free | FAB71442N-100UG | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7456APC | |||
| Biotin | NB7456B | |||
| DyLight 350 | NB7456UV | |||
| DyLight 405 | NB7456V | |||
| DyLight 488 | NB7456G | |||
| DyLight 550 | NB7456R | |||
| DyLight 594 | NB7456DL594 | |||
| DyLight 650 | NB7456C | |||
| DyLight 680 | NB7456FR | |||
| DyLight 755 | NB7456IR | |||
| HRP | NB7459 | |||
| Janelia Fluor 525 | NB7456JF525 | |||
| Janelia Fluor 669 | NB7456JF669 | |||
| PE | NB7456PE | |||
| PerCP | NB7456PCP | |||
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![N/A Kallikrein 5 [HRP]](http://images.novusbio.com/fullsize2/DATA_Kallikrein_5_DKK500_ELISA_731.jpg "N/A Kallikrein 5 [HRP]")
![N/A Kallikrein 5 [HRP]](http://images.novusbio.com/fullsize2/Kallikrein_5_DKK500_ELISA_125.jpg)
![ELISA TrkB [Biotin]](http://images.novusbio.com/fullsize2/dyc397-2_human-total-trkb-duoset-ic-elisa-5-plate-elisa-1592020151148.jpg "ELISA TrkB [Biotin]")
![N/A PSG1 [HRP]](http://images.novusbio.com/fullsize2/DATA_PSG1_DPSG10_ELISA_797.jpg "N/A PSG1 [HRP]")
![N/A PSG1 [HRP]](http://images.novusbio.com/fullsize2/PSG-1_DPSG10_ELISA_164.jpg)
![ELISA p53 [p Ser15] [Biotin]](http://images.novusbio.com/fullsize2/dyc1839-2_human-phospho-p53-s15-duoset-ic-elisa-5-plate-elisa-169202013376.jpg "ELISA p53 [p Ser15] [Biotin]")
![N/A MBL [HRP]](http://images.novusbio.com/fullsize2/DATA_MBL_DMBL00_ELISA_759.jpg "N/A MBL [HRP]")
![N/A MBL [HRP]](http://images.novusbio.com/fullsize2/MBL_DMBL00_ELISA_141.jpg)
![N/A Oncostatin M/OSM [HRP]](http://images.novusbio.com/fullsize2/DATA_OSM_MSM00_ELISA_1033.jpg "N/A Oncostatin M/OSM [HRP]")
![N/A Oncostatin M/OSM [HRP]](http://images.novusbio.com/fullsize2/OSM_MSM00_ELISA_2638.jpg)
![ELISA p21/CIP1/CDKN1A [Biotin]](http://images.novusbio.com/fullsize2/dyc1047-2_human-total-p21-cip1-cdkn1a-duoset-ic-elisa-2-plate-elisa-1592020122156.jpg "ELISA p21/CIP1/CDKN1A [Biotin]")