Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: 1.0 mg/ml

Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP] Summary

• Immunogen: Human IgE
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with human IgE. Cross-reactivity with other human immunoglobulins and light chains is less than 1%.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:2000 - 1:20000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2% BSA
• Preservative: 0.05% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.

Alternate Names for Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]

• Constant Region of Heavy Chain of IgE
• Ig Epsilon Chain C Region ND
• Ig Epsilon Chain C Region
• IgE
• Immunoglobulin Epsilon
• immunoglobulin heavy constant epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgE Heavy Chain Antibody (NB7459)(4)

Publications using NB7459

• Fu L, Wang C, Wang J et al. Maillard reaction with ribose, galacto-oligosaccharide or chitosan-oligosaccharide reduced the allergenicity of shrimp tropomyosin by inducing conformational changes Food Chem 2018-10-31 [PMID: 30373009] (ELISA)
• Perugino CA, AlSalem SB, Mattoo H et al. Identification of galectin-3 as an autoantigen in patients with IgG(4)-related disease J Allergy Clin Immunol 2018-06-01 [PMID: 29852256]
• Schelegle ES, Miller LA, Gershwin LJ et al. Repeated episodes of ozone inhalation amplifies the effects of allergen sensitization and inhalation on airway immune and structural development in Rhesus monkeys. Toxicol Appl Pharmacol. [PMID: 12915105]
  
  Details:
  Citation using the HRP form of this antibody.
• Schelegle ES, Gershwin LJ, Miller LA et al. Allergic asthma induced in rhesus monkeys by house dust mite (Dermatophagoides farinae). Am J Pathol. [PMID: 11141508]
  
  Details:
  Citation using the HRP form of this antibody.