HAF109). A specific band was detected for Relaxin-1 at approximately 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF109). A specific band was detected for Relaxin-1 at approximately 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pro-Relaxin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Relaxin-1 at approximately 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

MAB32571) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB32571) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

3257-RN) was serially diluted and captured by Rat Anti-Human Relaxin‑1 Monoclonal Antibody (Catalog # MAB32571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 3257-RN) was serially diluted and captured by Rat Anti-Human Relaxin‑1 Monoclonal Antibody (Catalog # MAB32571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF016). A specific band was detected for Prorelaxin-1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Prorelaxin-1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Relaxin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prorelaxin-1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human Relaxin‑1 protein was serially diluted 2-fold and captured by Rat Anti-Human Relaxin‑1 Monoclonal Antibody (Catalog # MAB32571) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

3257-RN) was serially diluted and captured by Rat Anti-Human Relaxin‑1 Monoclonal Antibody (Catalog # MAB32571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 3257-RN) was serially diluted and captured by Rat Anti-Human Relaxin‑1 Monoclonal Antibody (Catalog # MAB32571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Pro Relaxin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3257) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />