FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

HAF016). A specific band was detected for IL-7 Ra/CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for IL-7 Ra/CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of rat spleen tissue. PVDF Membrane was probed with 1 µg/mL of Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-7 Ra/CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

5-001-A) or (B) Sheep Anti-Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_lightbox"> 5-001-A) or (B) Sheep Anti-Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126)." class="big_thumb" />

MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="CD4⁺rat splenocytes were stained with Mouse Anti-Rat IL-7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB8484, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

747-MR) was serially diluted and captured by Rat Anti-Mouse IL-7R alpha/CD127 Monoclonal Antibody (Catalog # MAB4774) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-7R alpha/CD127 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 747-MR) was serially diluted and captured by Rat Anti-Mouse IL-7R alpha/CD127 Monoclonal Antibody (Catalog # MAB4774) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-7R alpha/CD127 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse IL-7R alpha/CD127 Fc Chimera (Catalog # 747-MR) was serially diluted and captured by Rat Anti-Mouse IL-7R alpha/CD127 Monoclonal Antibody (Catalog # MAB4774) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-7R alpha/CD127 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

207-IL/CF) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human IL-7R alpha/CD127 Fc Chimera Avi-tag (Catalog # AVI10317) binds with an ED₅₀ of 0.1-1 µg/mL." class="big_lightbox"> 207-IL/CF) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human IL-7R alpha/CD127 Fc Chimera Avi-tag (Catalog # AVI10317) binds with an ED₅₀ of 0.1-1 µg/mL." alt="When Recombinant Human IL-7 (Catalog # 207-IL/CF) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human IL-7R alpha/CD127 Fc Chimera Avi-tag (Catalog # AVI10317) binds with an ED₅₀ of 0.1-1 µg/mL." class="big_thumb" />

MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) is present at 500 ng/mL, Recombinant Human IL-7 (207-IL) binds with an ED₅₀ of 0.75-4.5 ng/mL." class="big_lightbox"> MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) is present at 500 ng/mL, Recombinant Human IL-7 (207-IL) binds with an ED₅₀ of 0.75-4.5 ng/mL." alt="In a His Tag Antibody (MAB050R) coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag (Catalog # 10758-IR) is present at 500 ng/mL, Recombinant Human IL-7 (207-IL) binds with an ED₅₀ of 0.75-4.5 ng/mL." class="big_thumb" />

207-IL), Recombinant Human IL-7R alpha/CD127 (Extracellular Domain) Fc Chimera Protein (Catalog # 10531-IR) binds with an ED₅₀ of 5-30 ng/mL." class="big_lightbox"> 207-IL), Recombinant Human IL-7R alpha/CD127 (Extracellular Domain) Fc Chimera Protein (Catalog # 10531-IR) binds with an ED₅₀ of 5-30 ng/mL." alt="In the presence of 100 ng/mL of Recombinant Human IL-7 (207-IL), Recombinant Human IL-7R alpha/CD127 (Extracellular Domain) Fc Chimera Protein (Catalog # 10531-IR) binds with an ED₅₀ of 5-30 ng/mL." class="big_thumb" />

10531-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-100 kDa and 156-200 kDa, respectively." class="big_lightbox"> 10531-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-100 kDa and 156-200 kDa, respectively." class="big_thumb" />