CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3166-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05531-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27073SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43995 | |||
| BSA Free | NBP2-52889-25ul | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining in human cerebral cortex and prostate tissues using NBP1-88648 antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP7/B-FABP Antibody [NBP1-88648] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP1-88648-img0013.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebellum shows strong cytoplasmic positivity in Bergmann glia.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0014.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0015.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human glioma shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0016.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0017.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP1-88648] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-88648-img0018.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP3-05531] - Western blot analysis of whole brain and neuron-glial cell culture lysates using FABP7/B-FABP Antibody at a at a dilution of of 1:1,000 in green, and chicken pAb to HSP60 at a dilution of of 1:10,000 in red: [1] protein standard, [2] adult rat brain, [3] embryonic (E18) rat brain, and [4] rat neuron-glial cells lysates. The bands at ~14kDa correspond to FABP7/B-FABP detected only in developing tissue and cells, and 60kDa bands represent HSP60 protein, which is labeled in all preparations.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP3-05531-img0002.jpg "Western Blot: FABP7/B-FABP Antibody [NBP3-05531] - Western blot analysis of whole brain and neuron-glial cell culture lysates using FABP7/B-FABP Antibody at a at a dilution of of 1:1,000 in green, and chicken pAb to HSP60 at a dilution of of 1:10,000 in red: [1] protein standard, [2] adult rat brain, [3] embryonic (E18) rat brain, and [4] rat neuron-glial cells lysates. The bands at ~14kDa correspond to FABP7/B-FABP detected only in developing tissue and cells, and 60kDa bands represent HSP60 protein, which is labeled in all preparations.")
![Immunocytochemistry/Immunofluorescence: FABP7/B-FABP Antibody [NBP3-05531] - Immunofluorescent analysis rat embryonic brain section stained with mouse FABP7/B-FABP at a at a dilution of of 1:500 in green, and costained with chicken pAb to vimentin at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. FABP7/B-FABP antibody stains developing astrocytes and radial glia, while vimentin antibody reveals cytoplasmic intermediate filaments. Since the sub cellular distribution of these two proteins is distinct they expression patterns overlap but are not identical.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05531-img0001.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-27073-img0004.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-27073-img0003.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BLBP antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-16399-img0005.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BLBP antibody [N1C3] diluted at 1:1000.")
![Immunohistochemistry-Frozen: FABP7/B-FABP Antibody [NBP2-16399] - Frozen sectioned E13.5 Rat brain. Green: BLBP protein stained by BLBP antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Frozen-NBP2-16399-img0004.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-16399] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-16399-img0002.jpg)
![FABP7 antibody [N1C3] detects FABP7 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FABP7 stained by FABP7 antibody [N1C3] (NBP2-16399) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16399_rabbit-polyclonal-fabp7-b-fabp-antibody-immunocytochemistry-immunofluorescence-2611202518283520.jpg)
![FABP7/B-FABP Antibody (CL0236) [NBP2-52889] - Analysis in human cerebral cortex and liver tissues using antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/nbp2-52889_mouse-monoclonal-fabp7-b-fabp-antibody-cl0236-immunohistochemistry-paraffin-284202311496..jpg "<b>Orthogonal Strategies Validation. </b> FABP7/B-FABP Antibody (CL0236) [NBP2-52889] - Analysis in human cerebral cortex and liver tissues using antibody. Corresponding FABP7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP7/B-FABP Antibody (CL0236) [NBP2-52889] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-CL0236-Western-Blot-NBP2-52889-img0003.jpg)
![FABP7/B-FABP Antibody (CL0236) [NBP2-52889] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/nbp2-52889_mouse-monoclonal-fabp7-b-fabp-antibody-cl0236-immunohistochemistry-paraffin-2842023115031..jpg)
![FABP7/B-FABP Antibody (CL0236) [NBP2-52889] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of cells in molecular layer.](http://images.novusbio.com/fullsize/nbp2-52889_mouse-monoclonal-fabp7-b-fabp-antibody-cl0236-immunohistochemistry-paraffin-2842023115157..jpg)
![FABP7/B-FABP Antibody (CL0236) [NBP2-52889] -Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/nbp2-52889_mouse-monoclonal-fabp7-b-fabp-antibody-cl0236-immunohistochemistry-paraffin-2842023115317..jpg)
![FABP7/B-FABP Antibody (CL0236) [NBP2-52889] -Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/nbp2-52889_mouse-monoclonal-fabp7-b-fabp-antibody-cl0236-immunohistochemistry-paraffin-2842023115442..jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP3-00016-img0005.jpg "Western Blot: FABP7/B-FABP Antibody [NBP3-00016] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human malignant melanoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP3-00016] - Immunochemical staining of human FABP7/B-FABP in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP3-00016-img0003.jpg)
![Immunoprecipitation: FABP7/B-FABP Antibody [NBP3-00016] - Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-FABP7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-FABP7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunoprecipitation-NBP3-00016-img0006.jpg)
![Western Blot: FABP7/B-FABP Antibody (PSH0-89) [NBP3-32084] - Western blot analysis of FABP7/B-FABP on different lysates with Rabbit anti-FABP7/B-FABP antibody (NBP3-32084) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: MCF7 cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32084) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32084_rabbit-fabp7-b-fabp-mab-psh0-89-27820241631648.jpg "Western Blot: FABP7/B-FABP Antibody (PSH0-89) [NBP3-32084] - Western blot analysis of FABP7/B-FABP on different lysates with Rabbit anti-FABP7/B-FABP antibody (NBP3-32084) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: MCF7 cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32084) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FABP7/B-FABP Antibody (PSH0-89) [NBP3-32084] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FABP7/B-FABP antibody (NBP3-32084) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32084) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32084_rabbit-fabp7-b-fabp-mab-psh0-89-29820241426263.jpg)
![ELISA: Human FABP7/B-FABP ELISA Kit (Colorimetric) [NBP2-82509] - Samples were spiked with high concentrations of Human FABP7/B-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82509_human-fabp7-b-fabp-elisa-kit-colorimetric-13220241617343.jpg "ELISA: Human FABP7/B-FABP ELISA Kit (Colorimetric) [NBP2-82509] - Samples were spiked with high concentrations of Human FABP7/B-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human FABP7/B-FABP ELISA Kit (Colorimetric) [NBP2-82509] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82509_human-fabp7-b-fabp-elisa-kit-colorimetric-432024915896.jpg)
![Western Blot: FABP7 Overexpression Lysate (Adult Normal) [NBL1-10423] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FABP7.](http://images.novusbio.com/fullsize/FABP7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10423-img0002.jpg "Western Blot: FABP7 Overexpression Lysate (Adult Normal) [NBL1-10423] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FABP7.")
![Western Blot: FABP7/B-FABP Antibody (PSH0-88) [NBP3-32083] - Western blot analysis of FABP7/B-FABP on different lysates with Rabbit anti-FABP7/B-FABP antibody (NBP3-32083) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: MCF7 cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32083) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32083_rabbit-fabp7-b-fabp-mab-psh0-88-278202415242665.jpg "Western Blot: FABP7/B-FABP Antibody (PSH0-88) [NBP3-32083] - Western blot analysis of FABP7/B-FABP on different lysates with Rabbit anti-FABP7/B-FABP antibody (NBP3-32083) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: MCF7 cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32083) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
