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![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0014.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0013.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0011.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human lymphoid tissues shows no positivity in non-germinal or germinal center cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0012.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in mouse brain.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0005.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in mouse brain.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in human colon.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0003.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in human placenta.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0004.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in transfected 293T cell line by CA1 polyclonal antibody.Lane 1: CA1 transfected lysate(28.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0006.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M05), clone M1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-M1-Western-Blot-H00000759-M05-img0007.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M05), clone M1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-M1-Western-Blot-H00000759-M05-img0005.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in human spleen.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-M1-Western-Blot-H00000759-M05-img0006.jpg)
![ELISA: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Detection limit for recombinant GST tagged CA1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-M1-ELISA-H00000759-M05-img0003.jpg)
![Immunoprecipitation: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 transfected lysate using anti-CA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CA1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-M1-Immunoprecipitation-H00000759-M05-img0004.jpg)
![Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-26220241525339.jpg "Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Positive Western Blot detected in: K562 whole cell lysate, U937 whole cell lysate. All lanes: Carbonic Anhydrase I/CA1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-282202411485846.jpg)
![Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Overlay Peak curve showing MCF-7 cells stained with Carbonic Anhydrase I/CA1 Antibody (26E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26469_rabbit-carbonic-anhydrase-i-ca1-mab-26e3-282202414183617.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591] - Western blot detection of Carbonic Anhydrase I/CA1 in Rat Brain lysates using NBP3-19591 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-S03-8F1-Western-Blot-NBP3-19591-img0002.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591] - Western blot detection of Carbonic Anhydrase I/CA1 in Rat Brain lysates using NBP3-19591 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591] - Immunohistochemistry of Carbonic Anhydrase I/CA1 in paraffin-embedded Human colon cancer tissue using NBP3-19591 at dilution 1/50](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-S03-8F1-Immunohistochemistry-Paraffin-NBP3-19591-img0001.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0005.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in human lung cancer.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0004.jpg)
![Sandwich ELISA: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Detection limit for recombinant GST tagged CA1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Sandwich-ELISA-H00000759-M10-img0003.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M07), clone 7G12. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-Western-Blot-H00000759-M07-img0004.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M07), clone 7G12. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Analysis of CA1 expression in human pancreas.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-Western-Blot-H00000759-M07-img0003.jpg)
![ELISA: Carbonic Anhydrase I/CA1 Antibody (7G12) [H00000759-M07] - Detection limit for recombinant GST tagged CA1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-7G12-ELISA-H00000759-M07-img0002.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-0G10F8-Western-Blot-NBP3-16404-img0001.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![ELISA: Human Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70050] - Samples were spiked with high concentrations of Human Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70050_human-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-132202416173419.jpg "ELISA: Human Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70050] - Samples were spiked with high concentrations of Human Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70050] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70050_human-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-432024941516.jpg)
![ELISA: Mouse Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70051] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70051_mouse-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-132202416211399.jpg "ELISA: Mouse Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70051] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70051] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70051_mouse-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-4320249192868.jpg)
![ELISA: Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70052] - Samples were spiked with high concentrations of Rat Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70052_rat-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-132202416211391.jpg "ELISA: Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70052] - Samples were spiked with high concentrations of Rat Carbonic Anhydrase I/CA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70052] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70052_rat-carbonic-anhydrase-i-ca1-elisa-kit-colorimetric-432024973660.jpg)
![Western Blot: Carbonic Anhydrase I Overexpression Lysate (Adult Normal) [NBL1-08609] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carbonic Anhydrase I.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08609-img0002.jpg "Western Blot: Carbonic Anhydrase I Overexpression Lysate (Adult Normal) [NBL1-08609] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carbonic Anhydrase I.")