| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-115SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98977-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98976-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89314-25ul | |||
![Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Knockdown-Validated-NB400-115-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NB400-115-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-115-img0005.jpg)
![Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Simple-Western-NB400-115-img0006.jpg)
![Western Blot: ABCF2 Antibody [NBP1-89316] - Analysis using Anti-ABCF2 antibody NBP1-89316 (A) shows similar pattern to independent antibody NBP1-89317 (B).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89316-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABCF2 Antibody [NBP1-89316] - Analysis using Anti-ABCF2 antibody NBP1-89316 (A) shows similar pattern to independent antibody NBP1-89317 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining in human testis and liver tissues using anti-ABCF2 antibody. Corresponding ABCF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0008.jpg)
![Western Blot: ABCF2 Antibody [NBP1-89316] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89316-img0005.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0006.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0007.jpg)
![Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP2-98977-img0003.jpg "Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa")
![Immunoprecipitation: ABCF2 Antibody [NBP2-98977] - ABCF2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 1 ul anti-ABCF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ABCF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunoprecipitation-NBP2-98977-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NBP2-98977] - Immunofluorescence staining of ABCF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98977 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98977_rabbit-polyclonal-abcf2-antibody-23520251125448.png)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP2-98976] - Immunochemical staining of human ABCF2 in human prostatic carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP2-98976-img0001.jpg "Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP2-98976] - Immunochemical staining of human ABCF2 in human prostatic carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Western Blot: ABCF1 Antibody [NBP1-89314] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-89314-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-89314] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ABCF1 Antibody [NBP1-89314] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89314-img0006.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-89314] - Analysis in human cell lines Caco-2 and MCF-7 using anti-ABCF1 antibody. Corresponding ABCF1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-89314-img0009.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0010.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0011.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0013.jpg)
![Western Blot: ABCF1 Antibody [NBP1-18889] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18889 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABC50 was also immunoprecipitated by NBP1-18888, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-18889-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-18889] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18889 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABC50 was also immunoprecipitated by NBP1-18888, which recognizes an upstream epitope.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: ABCF1 Antibody [NBP1-18889] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABC50 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-NBP1-18889-img0007.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-18889] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-ABC50 NBP1-18889 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-18889-img0004.jpg)
![Western Blot: ABCF1 Antibody [NBP2-15226] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP2-15226-img0003.jpg "Western Blot: ABCF1 Antibody [NBP2-15226] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE, antibody diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: ABCF1 Antibody [NBP2-15226] - Paraformaldehyde-fixed HeLa, using ABCF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15226-img0001.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP2-15226] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-15226-img0002.jpg)
![Western Blot: ABCF1 Antibody [NBP1-18888] - AWhole cell lysate from HeLa and 293T cells. ABC50 was also immunoprecipitated by another rabbit anti-ABC50 antibody.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-18888-img0005.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ABCF1 Antibody [NBP1-18888] - AWhole cell lysate from HeLa and 293T cells. ABC50 was also immunoprecipitated by another rabbit anti-ABC50 antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: ABCF1 Antibody [NBP1-18888] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ABC50 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-NBP1-18888-img0006.jpg)