Blogs for April 2019

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy

Tuesday, April 30, 2019 - 09:54
Alzheimer’s disease video Novus Biologicals

By Michalina Hanzel, PhD

In a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulation of misfolded, aggregate-prone proteins, as a result of a failure of the autophagy system, which leads to the loss of neuronal structure and function and eventual neuronal loss.

Autophagy is a conserved cellular process used to eliminate obsolete proteins and cell components by degradation and...

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity

Tuesday, April 23, 2019 - 08:28
IL10 receptor alpha expression in human colon carcinoma, IHC

By Jamshed Arslan, Pharm. D., PhD.

Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers.1 MMR-deficient colon cancer shows high CD8+ cytotoxic T-lymphocyte (CTL) and low Th17 cells, while MMR-proficient colon cancer shows an opposite trend. MMR-proficient colon cancer is associated with poor prognosis and is resistant to anti-cancerous immune checkpoint inhibitors. In other words, outcome of immune therapy is...

Visualizing Autophagy by Microscopy

Tuesday, April 16, 2019 - 09:36
LC3B  and alpha tubulin expression in HeLa cells treated with Chloroquine, ICC

By Christina Towers, PhD

Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to visualize and quantify the formation and turnover of these double membrane structures. Some techniques are more quantitative than others, each accompanied by advantages as well as caveats.

Identifying the autophagosome with Transmission Electron Microscopy (TEM)

This technique is the only tool that can reveal all of the dynamic stages of a forming autophagosome in the nanometer range and in its...

How to identify B cell subsets using flow cytometry

Tuesday, April 9, 2019 - 08:49
Explore new Flow Cytometry Handbook, Bio-Techne

By Victoria Osinski

Using flow cytometry to identify B cell subsets

Identifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified and isolated. While generally thought of as antibody-secreting cells, an expanding body of research proves that B cells are a heterogeneous population. Flow cytometry can be used to identify many of these B cell subsets, so long as important nuances and technical approaches are considered.

Setting up to identify B cell subsets

Before subsetting B cells, steps must be taken to ensure dead cells, cell aggregates, and debris are not...

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