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HAF018). A specific band was detected for SIRP alpha /CD172a at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B)." class="big_lightbox" target="_blank">
4670-CD) binds with an ED50 of 0.04-0.24 µg/mL." class="big_lightbox" target="_blank">
AVI4670) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human SIRP alpha His protein (Catalog # 9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_lightbox" target="_blank">
4670-CD) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRPa/CD172a Fc Chimera Avi-tag (Catalog # AVI4546) binds with an ED50 of 20-180 ng/mL." class="big_lightbox" target="_blank">
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).
![Western Blot SIRP alpha/CD172a Antibody (602411) [Unconjugated]](http://images.novusbio.com/images/antibody/mab4546_human-sirp-alpha-cd172a-mab-clone-602411-western-blot-2462021143010.jpg)
![N/A SIRP alpha/CD172a [Biotin]](http://images.novusbio.com/fullsize/dy4546-05_human-sirp-alpha-duoset-elisa-5-plate-278202113159.jpg)
![Bioactivity SIRP alpha/CD172a [Unconjugated]](http://images.novusbio.com/images/protein/SIRP_alpha_9378SA_1541.jpg)
![Bioactivity SIRP alpha/CD172a [Unconjugated]](http://images.novusbio.com/fullsize/protein/SIRP_alpha_7154SA_2310.jpg)
![Binding Activity SIRP alpha/CD172a [Biotin]](http://images.novusbio.com/images/protein/SIRP_alpha_AVI4546_3116.jpg)
![Flow Cytometry SIRP alpha/CD172a Antibody (602411) [Unconjugated]](http://images.novusbio.com/images/antibody/SIRP_alpha_MAB4546_Flow_Cytometry_10283.jpg)
![Immunocytochemistry SIRP alpha/CD172a Antibody (602411) [Unconjugated]](http://images.novusbio.com/images/antibody/SIRP_alpha_MAB4546_Immunocytochemistry_13035.jpg)
![Surface Plasmon Resonance (SPR) SIRP alpha/CD172a [Unconjugated]](http://images.novusbio.com/images/9378-sa_recombinant-human-sirp-alpha-cd172a-his-tag-protein-cf-surface-plasmon-resonance-spr-362024152756.jpg)
![SDS-Page SIRP alpha/CD172a [Biotin]](http://images.novusbio.com/images/protein/SIRP_alpha_AVI4546_3034.jpg)
