| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56583SS | |||
| Azide and BSA Free | NBP2-80872 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87758-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82074-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38305-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35166-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82069-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35466-20ul | |||
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Western-Blot-NB100-56583-img0004.jpg "Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0008.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0005.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0006.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Western-Blot-NBP1-87758-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87758-img0008.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Western-Blot-NBP1-87758-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human colon shows moderate cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human lymph node shows strong cytoplasmic positivity in germinal- and non-germinal center cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0015.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0016.jpg)
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0005.jpg "Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of U251 cells using NFkB p105/p50 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunocytochemistry-Immunofluorescence-NBP2-22178-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFkB p105/p50 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunohistochemistry-Paraffin-NBP2-22178-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Flow cytometric analysis of MCF-7 cells using NFkB p105/p50 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Flow-Cytometry-NBP2-22178-img0001.jpg)
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of A431 (A) and HeLa (B) cell lysate using NFkB p105/p50 (NBP2-22178) antibody at 1:500.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0008.jpg)
![ELISA: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-ELISA-NBP2-22178-img0004.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Western-Blot-NB100-82074-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Staining of methanol-fixed HeLa cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82074-img0005.jpg)
![Immunohistochemistry: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NB100-82074.](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-10320252332101.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Western blot analysis of extract from HeLa cells, using NB100-82074 (Lane 1 and 2).](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-103202523472637.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411545558.jpg "Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of A-549 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233811.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of U2OS cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572633.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411531043.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-10122024120610.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233849.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP3-38305] - Western blot analysis of lysates from Mouse lung, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572669.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123826.jpg "Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-35166] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFkB p105/p50 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: NFkB p105/p50 Antibody [NBP3-35166] - Western blot analysis of lysates from Raji cells, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414123838.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-35166] - Immunofluorescence analysis of MCF7 cells using NFkB p105/p50 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202415175235.jpg)
![Immunoprecipitation: NFkB p105/p50 Antibody [NBP3-35166] - Immunoprecipitation analysis of 150 ug extracts of MCF-7 cells using 3 ug NFkB p105/p50 antibody. Western blot was performed from the immunoprecipitate using NFkB p105/p50 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-35166_rabbit-nfkb-p105-p50-pab-912202414201377.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Staining of methanol-fixed Hela cells using NFkB-p105(Phospho-Ser927) Antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser927]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82069-img0007.jpg "Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Staining of methanol-fixed Hela cells using NFkB-p105(Phospho-Ser927) Antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Analysis of extracts from HL60 cells untreated or treated with TNF-alpha using NFkB-p105(Phospho-Ser927) antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser927]-Antibody-Western-Blot-NB100-82069-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82069(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82069_rabbit-polyclonal-nfkb-p105-p50-p-ser927-antibody-10320252346453.jpg)
![SDS-Page: Recombinant Human NFkB p105/p50 Protein [H00004790-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-NFkB-p105-p50-Protein-SDS-Page-H00004790-P01-img0002.jpg "SDS-Page: Recombinant Human NFkB p105/p50 Protein [H00004790-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - HeLa cell lysate using NBP2-61568.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-RM299-Western-Blot-NBP2-61568-img0003.jpg "Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - HeLa cell lysate using NBP2-61568.")
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - Staining of human tonsil tissue section using NBP2-61568.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-RM299-Immunohistochemistry-Paraffin-NBP2-61568-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-35466] - Immunofluorescence analysis of BALB-3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35466_rabbit-nfkb-p105-p50-pab-912202414154311.jpg "Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-35466] - Immunofluorescence analysis of BALB-3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NFkB p105/p50 Antibody [NBP3-35466] - Western blot analysis of lysates from Rat testis, using NFkB p105/p50 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35466_rabbit-nfkb-p105-p50-pab-912202415111877.jpg)
![Western Blot: NFkB p105/p50 Overexpression Lysate (Adult Normal) [NBL1-13619] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFkB p105/p50.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13619-img0002.jpg "Western Blot: NFkB p105/p50 Overexpression Lysate (Adult Normal) [NBL1-13619] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFkB p105/p50.")
![Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33571_rabbit-nfkb-p105-p50-p-ser932-mab-8b9g6-101220241233843.jpg "Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33571_rabbit-nfkb-p105-p50-p-ser932-mab-8b9g6-101220241224153.jpg)