HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog #
MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." class="big_lightbox">
HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog #
MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane (left) was probed with 0.1 µg/mL of Mouse Anti-Human ATG12 Monoclonal Antibody (Catalog # MAB6807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog #
MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." class="big_thumb" />
HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane (left) was probed with 0.1 µg/mL of Mouse Anti-Human ATG12 Monoclonal Antibody (Catalog # MAB6807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane (right) was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the ATG5:ATG12 heterodimer at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0015.jpg "Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG12 Antibody [NBP2-15501] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0011.jpg)
![Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0014.jpg)
![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0008.jpg)
![Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunoprecipitation-NBP2-15501-img0006.jpg)
![Western Blot: ATG12 Antibody [NBP2-15501] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15501_rabbit-polyclonal-atg12-antibody-western-blot-81202481244..jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for ![Immunocytochemistry/Immunofluorescence: ATG12 Antibody (166) [NBP2-43781] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [166]. Red: Phalloidin, a F-actin marker.](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Immunocytochemistry-Immunofluorescence-NBP2-43781-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATG12 Antibody (166) [NBP2-43781] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [166]. Red: Phalloidin, a F-actin marker.")
![<b>Biological Strategies Validation. </b>Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Western-Blot-NBP2-43781-img0001.jpg)
![Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-166-Western-Blot-NBP2-43781-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] (NBP2-43781) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43781_mouse-monoclonal-atg12-antibody-166-western-blot-2611202518411322.jpg)
![Western Blot: ATG12 Antibody [NBP1-76860] - Human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP1-76860-img0003.jpg "Western Blot: ATG12 Antibody [NBP1-76860] - Human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody - BSA Free [NBP1-76860] - Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76860_rabbit-polyclonal-atg12-antibody-271120241918612.jpg)
![Immunohistochemistry: ATG12 Antibody - BSA Free [NBP1-76860] - Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76860_rabbit-polyclonal-atg12-antibody-2711202420272493.jpg)
![Western Blot: ATG12 Antibody [NBP1-76861] - Mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP1-76861-img0003.jpg "Western Blot: ATG12 Antibody [NBP1-76861] - Mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: ATG12 Antibody - BSA Free [NBP1-76861] - Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76861_rabbit-polyclonal-atg12-antibody-271120242004118.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody - BSA Free [NBP1-76861] - Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76861_rabbit-polyclonal-atg12-antibody-2711202420141980.jpg)
![Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-9411-Western-Blot-NBP2-43753-img0001.jpg "Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000")
![Western Blot: ATG12 Antibody (9411) [NBP2-43753] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [9411] dilution: 1:1000.](http://images.novusbio.com/fullsize/ATG12-Antibody-9411-Western-Blot-NBP2-43753-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT9411] (NBP2-43753) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43753_mouse-monoclonal-atg12-antibody-9411-western-blot-261120251943817.jpg)
![Western Blot: ATG12 Antibody (948) [NBP2-43752] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [948] dilution: 1:1000](http://images.novusbio.com/fullsize/ATG12-Antibody-948-Western-Blot-NBP2-43752-img0001.jpg "Western Blot: ATG12 Antibody (948) [NBP2-43752] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [948] dilution: 1:1000")
![Western Blot: ATG12 Antibody (948) [NBP2-43752] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [948] dilution: 1:1000.](http://images.novusbio.com/fullsize/ATG12-Antibody-948-Western-Blot-NBP2-43752-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT948] (NBP2-43752) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43752_mouse-monoclonal-atg12-antibody-948-western-blot-261120251822201.jpg)
![Western Blot: ATG12 Antibody [NB600-603] - Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NB600-603-img0002.jpg "Western Blot: ATG12 Antibody [NB600-603] - Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.")
![N/A: ATG12 Antibody [NB600-603]](http://images.novusbio.com/fullsize/ATG12-Antibody-N-A-NB600-603-img0003.jpg)
![Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-278202416285257.jpg "Western Blot: ATG12 Antibody (JE30-61) [NBP3-32056] - Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HEK-293 cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (JE30-61) [NBP3-32056] - Immunocytochemistry analysis of HCT 116 cells labeling ATG12 with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATG12 antibody (NBP3-32056) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-2920241192327.jpg)
![Flow Cytometry: ATG12 Antibody (JE30-61) [NBP3-32056] - Flow cytometric analysis of HCT 116 cells labeling ATG12. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32056, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32056_rabbit-atg12-mab-je30-61-29202416453286.jpg)
![Western Blot: ATG12 Antibody (S08-2F4) [NBP3-15002] - Western blot detection of K562, 3T3, Hela cell lysates using NBP3-15002. Predicted band size: 15kDa. Observed band size: 55kDa.](http://images.novusbio.com/fullsize/nbp3-15002_rabbit-atg12-mab-s08-2f4-103202523472631.jpg "Western Blot: ATG12 Antibody (S08-2F4) [NBP3-15002] - Western blot detection of K562, 3T3, Hela cell lysates using NBP3-15002. Predicted band size: 15kDa. Observed band size: 55kDa.")
![Western Blot: ATG12 Antibody (9R8W2) [NBP3-15812] - Western blot analysis of extracts of various cell lines, using ATG12 antibody (NBP3-15812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ATG12-Antibody-9R8W2-Western-Blot-NBP3-15812-img0002.jpg "Western Blot: ATG12 Antibody (9R8W2) [NBP3-15812] - Western blot analysis of extracts of various cell lines, using ATG12 antibody (NBP3-15812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
