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![ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-ELISA-NBP3-12167-img0001.jpg "ELISA: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - ELISA Results of RFP antibody (RFP40) reactive against RFP. The working dilution for clone RFP40 is 1:31000. Each well was coated in duplicate with 1.0ug of RFP. The starting dilution of antibody was 45 ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS blocking buffer. Mouse Anti-FLAG Tag HRP conjugated Antibody and TMB substrate.")
![Dot Blot: RFP Antibody (RFP40) - VHH - BSA Free [NBP3-12167] - Row A: Recombinant RFP protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.0hr at RT. Secondary Antibody: Mouse Anti-Flag Peroxidase at 1:5000 for 30 mins at RT. Block: BlockOut Buffer.. Exposure: 10 seconds.](http://images.novusbio.com/fullsize/RFP-Antibody-RFP40-VHH-BSA-Free-Dot-Blot-NBP3-12167-img0002.jpg)
![Western Blot Results of RFP Antibody (RFP40) - VHH - BSA Free. Lane 1: Opal Prestained Molecular Weight . Lane 2: HEK293 Whole Cell Lysate [10ug] (-). Lane 3: HEK293 Whole Cell Lysate [10ug] + GFP rec. protein [1ug] (-). Lane 4: HEK293 Whole Cell Lysate [10ug] + RFP rec. protein [1ug] (+). Lane 5: RFP rec. protein [1ug] (+).Primary Antibody: RFP Antibody (RFP40) - VHH - BSA Free at 1:1000 for overnight at 4°C. Secondary Antibody: Mouse Anti-6x His DL649 conjugated at 1:10,000 for 1hr at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4°C. Expected MW: ~27kDa for RFP protein.](http://images.novusbio.com/fullsize/nbp3-12167_llama-monoclonal-rfp-antibody-rfp40-187202581873.jpg)
![Western Blot: RNF41 Antibody [NBP2-20191] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/RNF41-Antibody-Western-Blot-NBP2-20191-img0001.jpg "Western Blot: RNF41 Antibody [NBP2-20191] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: RNF41 Antibody [NBP2-20191] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF41-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20191-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF41 Antibody [NBP2-20191] - Human colon carcinoma. RNF41 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RNF41-Antibody-Immunohistochemistry-Paraffin-NBP2-20191-img0003.jpg)
![Western Blot: RNF25 Antibody [NBP1-31491] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF25-Antibody-Western-Blot-NBP1-31491-img0004.jpg "Western Blot: RNF25 Antibody [NBP1-31491] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RNF25 Antibody [NBP1-31491] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RNF25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31491-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF25 Antibody [NBP1-31491] - HeLa xenograft. RNF25 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RNF25-Antibody-Immunohistochemistry-Paraffin-NBP1-31491-img0003.jpg)
![Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.](http://images.novusbio.com/fullsize/RFP-Antibody-Immunoprecipitation-NBP2-19302-img0003.jpg "Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.")
![Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19302_rabbit-polyclonal-rfp-antibody-9102024207593.jpg)
![Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20806] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20806-img0003.jpg "Immunocytochemistry/Immunofluorescence: UHRF1 Antibody [NBP2-20806] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UHRF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: UHRF1 Antibody [NBP2-20806] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/UHRF1-Antibody-Immunohistochemistry-Paraffin-NBP2-20806-img0002.jpg)
![Western Blot: RNF133 Antibody [NBP2-20176] - Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF133-Antibody-Western-Blot-NBP2-20176-img0001.jpg "Western Blot: RNF133 Antibody [NBP2-20176] - Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RNF133 Antibody [NBP2-20176] - Paraffin-embedded human endometrial cancer. RNF133 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RNF133-Antibody-Immunohistochemistry-Paraffin-NBP2-20176-img0004.jpg)
![Immunohistochemistry-Paraffin: RNF133 Antibody [NBP2-20176] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF133-Antibody-Immunohistochemistry-Paraffin-NBP2-20176-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF133 Antibody [NBP2-20176] - Paraffin-embedded human colon cancer. RNF133 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RNF133-Antibody-Immunohistochemistry-Paraffin-NBP2-20176-img0003.jpg)
![Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Western-Blot-NBP1-32050-img0009.jpg "Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-32050] - HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32050-img0006.jpg)
![Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-32050] - Human breast cancer, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRIM25-Antibody-Immunohistochemistry-Paraffin-NBP1-32050-img0002.jpg)
![Western Blot: RNF13 Antibody [NBP1-31251] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF13-Antibody-Western-Blot-NBP1-31251-img0004.jpg "Western Blot: RNF13 Antibody [NBP1-31251] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RNF13 Antibody [NBP1-31251] - Paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF13-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31251-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF13 Antibody [NBP1-31251] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF13-Antibody-Immunohistochemistry-Paraffin-NBP1-31251-img0003.jpg)
![Western Blot: VPS11 Antibody [NBP1-31054] - Sample(30 ug of whole cell lysate)A:293TB:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VPS11-Antibody-Western-Blot-NBP1-31054-img0003.jpg "Western Blot: VPS11 Antibody [NBP1-31054] - Sample(30 ug of whole cell lysate)A:293TB:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: VPS11 Antibody [NBP1-31054] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VPS11 protein stained by VPS11 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/VPS11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31054-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054] - VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. VPS11 antibody (NBP1-31054).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/VPS11-Antibody-Immunohistochemistry-Paraffin-NBP1-31054-img0004.jpg)
![Western Blot: MNAB Antibody [NBP1-55060] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/MNAB-Antibody-Western-Blot-NBP1-55060-img0008.jpg "Western Blot: MNAB Antibody [NBP1-55060] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.")
![Western Blot: MNAB Antibody [NBP1-55060] - Human Adult Placenta.](http://images.novusbio.com/fullsize/MNAB-Antibody-Western-Blot-NBP1-55060-img0003.jpg)
![Western Blot: MNAB Antibody [NBP1-55060] - Human Fetal Lung.](http://images.novusbio.com/fullsize/MNAB-Antibody-Western-Blot-NBP1-55060-img0006.jpg)
![Western Blot: MNAB Antibody [NBP1-55060] - PANC1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MNAB-Antibody-Western-Blot-NBP1-55060-img0002.jpg)
![Western Blot: MNAB Antibody [NBP1-55060] - Analysis of 721_B cell lysate. Antibody Dilution: 1.0 ug/ml RC3H2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/MNAB-Antibody-Western-Blot-NBP1-55060-img0007.jpg)
![Western Blot: TRAF7 Antibody [NBP1-58999] - Human Stomach, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/TRAF7-Antibody-Western-Blot-NBP1-58999-img0002.jpg "Western Blot: TRAF7 Antibody [NBP1-58999] - Human Stomach, concentration 0.2-1 ug/ml.")
![Western Blot: TRIM9 Antibody (2A1) [NBP2-46233] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9.](http://images.novusbio.com/fullsize/TRIM9-Antibody-2A1-Western-Blot-NBP2-46233-img0004.jpg "Western Blot: TRIM9 Antibody (2A1) [NBP2-46233] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9.")
![Flow Cytometry: TRIM9 Antibody (2A1) [NBP2-46233] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by TRIM9 antibody.](http://images.novusbio.com/fullsize/TRIM9-Antibody-2A1-Flow-Cytometry-NBP2-46233-img0003.jpg)
![Flow Cytometry: TRIM9 Antibody (2A1) [NBP2-46233] - Analysis of Hela cells, using TRIM9 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRIM9-Antibody-2A1-Flow-Cytometry-NBP2-46233-img0001.jpg)
![Flow Cytometry: TRIM9 Antibody (2A1) [NBP2-46233] - Analysis of Jurkat cells, using TRIM9 antibody,(Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TRIM9-Antibody-2A1-Flow-Cytometry-NBP2-46233-img0002.jpg)