| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05396-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47742-0.1mg | |||
| IHC-Prediluted | NBP2-48217 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54330-100ug | |||
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13266-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13266-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13266-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13267-img0002.jpg "Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunohistochemistry-Paraffin-NBP3-13267-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13229-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13229-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13229-img0003.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
![Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Src [p Tyr529] Antibody-Western Blot-NBP3-05396-img0001.jpg "Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Src [p Tyr529] Antibody - Azide and BSA Free [Src] - Western blot analysis of lysates from A-431 cells, using Src Rabbit pAb at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-05396_rabbit-polyclonal-src-p-tyr529-antibody-52202516293989.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241252814.jpg "Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: PTP epsilon Antibody [NBP3-38398] - Western blot analysis of various lysates using PTP epsilon Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-1012202411531092.jpg)
![Immunohistochemistry: PTP epsilon Antibody [NBP3-38398] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PTP epsilon Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38398_rabbit-ptp-epsilon-pab-101220241224188.jpg)
![Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-278202415295551.jpg "Western Blot: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Western blot analysis of HGFR/c-MET on different lysates with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32199) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-HGFR/c-MET antibody (NBP3-32199) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32199) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-298202413585510.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (PSH0-19) [NBP3-32199] - Flow cytometric analysis of Hela cells labeling HGFR/c-MET. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32199, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32199_rabbit-hgfr-c-met-mab-psh0-19-29202416453259.jpg)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR1195) [NBP2-45150] - Human Placenta stained with EGFR Monoclonal Antibody (GFR1195).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR1195-Immunohistochemistry-Paraffin-NBP2-45150-img0001.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (GFR1195) [NBP2-45150] - Human Placenta stained with EGFR Monoclonal Antibody (GFR1195).")
![Protein Array: EGFR Antibody (GFR1195) [NBP2-45150] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (GFR1195) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR1195-Protein-Array-NBP2-45150-img0003.jpg)
![Western Blot: EGFR Antibody (GFR/1708) [NBP2-53198] - Western Blot Analysis of A431 cell lysate using EGFR antibody (GFR/1708).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1708-Western-Blot-NBP2-53198-img0004.jpg "Western Blot: EGFR Antibody (GFR/1708) [NBP2-53198] - Western Blot Analysis of A431 cell lysate using EGFR antibody (GFR/1708).")
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR/1708) [NBP2-53198] - Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Antibody (GFR/1708).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1708-Immunohistochemistry-Paraffin-NBP2-53198-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR/1708) [NBP2-53198] - Formalin-fixed, paraffin-embedded human Lung SCC stained with EGFR Monoclonal Antibody (GFR/1708).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR-1708-Immunohistochemistry-Paraffin-NBP2-53198-img0001.jpg)