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![Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/Insulin-Receptor-Beta-[p-Thr1160]-Antibody-Western-Blot-NBP2-60777-img0001.jpg "Western Blot: Insulin Receptor Beta [p Thr1160] Antibody [NBP2-60777] - Western blot of HeLa cell lysate showing specific labeling of the~95kDa IR protein phosphorylated at Thr1160 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Human brain tissue lysate (40 ug/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33070_rabbit-dtk-tyro3-mab-je38-13-288202414191540.jpg "Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Human brain tissue lysate (40 ug/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody [NBP2-16970] - Analysis of paraffin-embedded human lung cancer, using Insulin Receptor antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-Immunohistochemistry-Paraffin-NBP2-16970-img0004.jpg "Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody [NBP2-16970] - Analysis of paraffin-embedded human lung cancer, using Insulin Receptor antibody at 1:250 dilution.")
![Western Blot: CD3 zeta Antibody [NBP2-15796] - Analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-Western-Blot-NBP2-15796-img0001.jpg "Western Blot: CD3 zeta Antibody [NBP2-15796] - Analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-p-Thr669-Antibody-GT1361-Western-Blot-NBP3-13645-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: YAP1 [p Ser61] Antibody [NBP3-13319] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 (phospho Ser61) antibody (NBP3-13319) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/YAP1-[p-Ser61]-Antibody-Western-Blot-NBP3-13319-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: YAP1 [p Ser61] Antibody [NBP3-13319] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 (phospho Ser61) antibody (NBP3-13319) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1047--p-Ser1046]-Antibody-Western-Blot-NBP3-13230-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1047, p Ser1046] Antibody [NBP3-13230] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Thr669]-Antibody-Western-Blot-NBP3-13226-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody [NBP3-13226] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13226) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Src [p Tyr416] Antibody [NBP3-13419] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr416) antibody (NBP3-13419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Src-[p-Tyr416]-Antibody-Western-Blot-NBP3-13419-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Src [p Tyr416] Antibody [NBP3-13419] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr416) antibody (NBP3-13419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr950]-Antibody-Western-Blot-NBP3-13235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")