| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37326-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-248SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05933-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98661-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89383-50ul | |||
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0004.jpg "Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.")
![Flow Cytometry: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Flow-Cytometry-NBP2-37326-img0005.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0002.jpg)
![Western Blot: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-Western-Blot-NBP2-37326-img0003.jpg)
![ELISA: EGF R/ErbB1 Antibody (5E10D3) [NBP2-37326] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-5E10D3-ELISA-NBP2-37326-img0001.jpg)
![Western Blot: NORE1 Antibody [NB500-248] - Analysis of NORE1 using this antibody at 1:1000 dilution on 293 T cell lysates overexpressed with NORE1.](http://images.novusbio.com/fullsize/NORE1-Antibody-Western-Blot-NB500-248-img0002.jpg "Western Blot: NORE1 Antibody [NB500-248] - Analysis of NORE1 using this antibody at 1:1000 dilution on 293 T cell lysates overexpressed with NORE1.")
![Western Blot: 26S proteasome subunit 9 Antibody [NBP3-05933] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:28 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/26S-proteasome-subunit-9-Antibody-Western-Blot-NBP3-05933-img0001.jpg "Western Blot: 26S proteasome subunit 9 Antibody [NBP3-05933] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:28 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP3-05933] - Staining of human PSMD9 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/26S-proteasome-subunit-9-Antibody-Immunohistochemistry-Paraffin-NBP3-05933-img0003.jpg)
![Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP3-05933] - Staining of human PSMD9 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/26S-proteasome-subunit-9-Antibody-Immunohistochemistry-Paraffin-NBP3-05933-img0002.jpg)
![Immunoprecipitation: 26S proteasome subunit 9 Antibody [NBP3-05933] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PSMD9 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMD9 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/26S-proteasome-subunit-9-Antibody-Immunoprecipitation-NBP3-05933-img0004.jpg)
![Western Blot: Aprataxin Antibody [NBP2-98661] - Anti-Aprataxin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/Aprataxin-Antibody-Western-Blot-NBP2-98661-img0004.jpg "Western Blot: Aprataxin Antibody [NBP2-98661] - Anti-Aprataxin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661] - Immunochemical staining of human Aprataxin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Aprataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-98661-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Aprataxin Antibody [NBP2-98661] - Immunofluorescence staining of Aprataxin in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98661 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-98661_rabbit-polyclonal-aprataxin-antibody-235202521451772.png)
![Immunohistochemistry-Paraffin: Aprataxin Antibody - BSA Free [NBP2-98661] - Immunochemical staining of human Aprataxin in human brain with NBP2-98661 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-98661_rabbit-polyclonal-aprataxin-antibody-235202514331580.png)
![Immunohistochemistry-Paraffin: Endostatin Antibody (1837.46) [NBP1-40051] - Analysis of anti-COL18A1 / Endostatin antibody with human kidney at dilution 1:100.](http://images.novusbio.com/fullsize/Endostatin-Antibody-1837-46-Immunohistochemistry-Paraffin-NBP1-40051-img0001.jpg "Immunohistochemistry-Paraffin: Endostatin Antibody (1837.46) [NBP1-40051] - Analysis of anti-COL18A1 / Endostatin antibody with human kidney at dilution 1:100.")
![Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Western-Blot-NBP2-89383-img0003.jpg "Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383] - Staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunocytochemistry-Immunofluorescence-NBP2-89383-img0001.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383] - Analysis of Human Galectin3 expression on HeLa cells. The cells were treated stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Flow-Cytometry-NBP2-89383-img0002.jpg)
![Immunoprecipitation: Galectin-3 Antibody (024) [NBP2-89383] - Lane A:0.5 mg mouse pancreas Whole Cell Lysate 4 uL anti-NKX3-1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-NKX3-1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.Predicted band size: 27 kDa Observed band size :30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunoprecipitation-NBP2-89383-img0004.jpg)
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-298202414262699.jpg "Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunocytochemistry analysis of MCF-7 cells labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202410234277.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Flow cytometric analysis of SK-Br-3 cells labeling ErbB2/Her2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32422, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202416453290.jpg)
![Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: HL-60 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 26 kDa<br/> Observed band size: 26 kDa<br/><br/> Exposure time: 1 minute 40 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-278202415212474.jpg "Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: HL-60 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 26 kDa<br/> Observed band size: 26 kDa<br/><br/> Exposure time: 1 minute 40 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-298202414333630.jpg)
![Flow Cytometry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Flow cytometric analysis of THP-1 cells labeling Bcl-2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32072, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-29202416372125.jpg)
![Western blot analysis on different lysates with NBP3-32072 at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32072 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_-western-blot-639204590078307079.jpg)
![Immunohistochemistry: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32073) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32073_rabbit-bcl-2-mab-pd01-41-29820241430412.jpg "Immunohistochemistry: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32073) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunocytochemistry analysis of HeLa cells labeling Bcl-2 with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32073_rabbit-bcl-2-mab-pd01-41-29202410234278.jpg)
![Western blot analysis on different lysates with NBP3-32073 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: HL-60 cell lysate (15 µg/Lane) Lane 4: THP-1 cell lysate (15 µg/Lane) Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32073 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32073_-western-blot-639204594552076536.jpg)
![Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-278202415212443.jpg "Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: c-Myc Antibody (PS00-11) [NBP3-32201] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-c-Myc antibody (NBP3-32201) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32201) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-298202413585552.jpg)
![Western blot analysis on different lysates with NBP3-32201 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32201 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_-western-blot-639204594550909475.jpg)
![Western blot analysis on different lysates with NBP3-32201 at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: Neuro-2a cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32201 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_-western-blot-639204597794751464.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western blot analysis on different lysates with NBP3-32698 at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32698 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_-western-blot-639204594545626873.jpg)
![Western blot analysis on different lysates with NBP3-32698 at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: Saos-2 cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32698 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_-western-blot-639204597793269876.jpg)