| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89515-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95281-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09045-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12025-0.025mg | |||
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32754_rabbit-chk1-p-ser280-mab-je47-66-288202412442937.jpg "Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_mouse-erk2-mab-5-d2-r-278202415295598.jpg "Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: BID Antibody (002) [NBP2-89515] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:22 kDa](http://images.novusbio.com/fullsize/BID-Antibody-002-Western-Blot-NBP2-89515-img0002.jpg "Western Blot: BID Antibody (002) [NBP2-89515] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:22 kDa")
![Immunoprecipitation: BID Antibody (002) [NBP2-89515] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg RAW264.7 Whole Cell Lysate 2 uL anti-BID rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-BID rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/BID-Antibody-002-Immunoprecipitation-NBP2-89515-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody [NBP2-95281] - Staining of human p63/TP73L in human skin with rabbit polyclonal antibody (1:2000).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-Immunohistochemistry-Paraffin-NBP2-95281-img0002.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody [NBP2-95281] - Staining of human p63/TP73L in human skin with rabbit polyclonal antibody (1:2000).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody [NBP2-95281] - Staining of human p63/TP73L in human prostate with rabbit polyclonal antibody (1:2000).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-Immunohistochemistry-Paraffin-NBP2-95281-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (2C8) [H00005292-M02] - Analysis of monoclonal antibody to PIM1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIM1-Antibody-2C8-Immunocytochemistry-Immunofluorescence-H00005292-M02-img0002.jpg "Immunocytochemistry/Immunofluorescence: PIM1 Antibody (2C8) [H00005292-M02] - Analysis of monoclonal antibody to PIM1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23435_rabbit-bad-p-ser112-pab-19920231410131.jpg "Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).")
![Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.](http://images.novusbio.com/fullsize/nbp3-23345_rabbit-profilin-1-p-tyr129-pab-19920231856966.jpg "Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.")
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-45125_rabbit-p16ink4a-cdkn2a-mab-30859-s17-chimeric-bsa-free-immunocytochemistry-immunofluorescence-222026035554.png "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: TWEAK/TNFSF12 Antibody (MTW-1) - Chimeric [NBP3-09045] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MTW-1 (NBP3-09045) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09045, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-MTW-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09045-img0001.jpg "Immunocytochemistry/Immunofluorescence: TWEAK/TNFSF12 Antibody (MTW-1) - Chimeric [NBP3-09045] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MTW-1 (NBP3-09045) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09045, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric [NBP3-12025] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of chi220 (NBP3-12025) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12025, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-chi220-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12025-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric [NBP3-12025] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of chi220 (NBP3-12025) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12025, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free [NBP3-12025] - Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12025 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12025_rabbit-monoclonal-cd40-ligand-tnfsf5-antibody-chi220-chimeric-74202522113024.png)