| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90283-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98571-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98809-50ul | |||
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0005.jpg "Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).")
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg "Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Western blot analysis on different lysates with NBP3-32782 at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5μM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5μM Doxorubicin for 24 hours, then treated with lambda pp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32782 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_-western-blot-639204595248046518.jpg)
![Immunohistochemistry: Bcl-6 Antibody (A6C8-R) [NBP3-32075] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32075) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32075) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32075_mouse-bcl-6-mab-a6c8-r-29820241492688.jpg "Immunohistochemistry: Bcl-6 Antibody (A6C8-R) [NBP3-32075] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32075) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32075) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody (A6C8-R) [NBP3-32075] - Immunocytochemistry analysis of Raji cells labeling Bcl-6 with Mouse anti-Bcl-6 antibody (NBP3-32075) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Bcl-6 antibody (NBP3-32075) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32075_mouse-bcl-6-mab-a6c8-r-2920241192338.jpg)
![Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_-western-blot-639204590075999270.jpg "Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_-western-blot-639204597793077982.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)
![Western Blot: FHIT Antibody (104) [NBP2-90283] - Anti-FHIT rabbit monoclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa.](http://images.novusbio.com/fullsize/FHIT-Antibody-104-Western-Blot-NBP2-90283-img0001.jpg "Western Blot: FHIT Antibody (104) [NBP2-90283] - Anti-FHIT rabbit monoclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa.")
![Immunocytochemistry/Immunofluorescence: FHIT Antibody (104) [NBP2-90283] - Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90283 antibody (1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-90283_rabbit-monoclonal-fhit-antibody-104-235202514371347.png)
![Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: A549 treated with pp for 1 hour cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 110/140 kDa<br/><br/> Exposure time: 57 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_rabbit-mdm2-hdm2-p-ser166-mab-je64-20-288202412461655.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: A549 treated with pp for 1 hour cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 110/140 kDa<br/><br/> Exposure time: 57 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-32777 at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with 1μg/mL insulin for 2.5 hours cell lysate Lane 3: A549 treated with 1μg/mL insulin for 2.5 hours cell lysate, then the membrane treated with lambda pp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32777 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_-western-blot-639204597138504533.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32791_rabbit-pten-p-ser380-mab-jj08-81-2920241192374.jpg "Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-98571-img0003.jpg "Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-98571] - Immunochemical staining of human PRP19 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-98571-img0002.jpg)
![Western Blot: KLF13 Antibody [NBP2-98809] - Anti-KLF13 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/KLF13-Antibody-Western-Blot-NBP2-98809-img0002.jpg "Western Blot: KLF13 Antibody [NBP2-98809] - Anti-KLF13 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa")
![Immunoprecipitation: KLF13 Antibody [NBP2-98809] - KLF13 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate1 ul anti-KLF13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-KLF13 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/KLF13-Antibody-Immunoprecipitation-NBP2-98809-img0001.jpg)
![Immunohistochemistry-Paraffin: Kallikrein 15 Antibody (02) - Azide and BSA Free [NBP3-26079] - Immunochemical staining of human Kallikrein 15 in human testis with NBP3-26079 at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26079_mouse-kallikrein-15-mab-02-azide-and-bsa-free-12620241540467.png "Immunohistochemistry-Paraffin: Kallikrein 15 Antibody (02) - Azide and BSA Free [NBP3-26079] - Immunochemical staining of human Kallikrein 15 in human testis with NBP3-26079 at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Kallikrein 15 Antibody (02) - Azide and BSA Free [NBP3-26079] - Immunochemical staining of human Kallikrein 15 in human stomach with NBP3-26079 at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26079_mouse-kallikrein-15-mab-02-azide-and-bsa-free-12620241351414.png)
![Immunohistochemistry-Paraffin: Kallikrein 15 Antibody (02) - Azide and BSA Free [NBP3-26079] - Immunochemical staining of human Kallikrein 15 in human prostate with NBP3-26079 at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-26079_mouse-kallikrein-15-mab-02-azide-and-bsa-free-1262024149348.png)