| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99342-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99356-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98763-50ul | |||
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-87905-img0003.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0002.jpg)
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Smooth Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0001.jpg)
![Western Blot: NFYA Antibody [NBP2-87908] - WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-87908-img0003.jpg "Western Blot: NFYA Antibody [NBP2-87908] - WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells")
![Immunohistochemistry: NFYA Antibody [NBP2-87908] - HumanMuscle](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87908-img0002.jpg)
![Immunohistochemistry: NFYA Antibody [NBP2-87908] - Human Liver](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87908-img0001.jpg)
![Western Blot: JMJD2D Antibody [NBP2-87645] - WB Suggested Anti-JMJD2D Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain](http://images.novusbio.com/fullsize/JMJD2D-Antibody-Western-Blot-NBP2-87645-img0003.jpg "Western Blot: JMJD2D Antibody [NBP2-87645] - WB Suggested Anti-JMJD2D Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain")
![Immunohistochemistry: JMJD2D Antibody [NBP2-87645] - Human kidney](http://images.novusbio.com/fullsize/JMJD2D-Antibody-Immunohistochemistry-NBP2-87645-img0002.jpg)
![Immunohistochemistry: JMJD2D Antibody [NBP2-87645] - Human Intestine](http://images.novusbio.com/fullsize/JMJD2D-Antibody-Immunohistochemistry-NBP2-87645-img0001.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0003.jpg "Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90081 antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).](http://images.novusbio.com/fullsize/nbp2-90081_rabbit-monoclonal-hsf1-antibody-009-235202514492390.png)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-99342] - Immunochemical staining of human E6AP/UBE3A in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP2-99342-img0003.jpg "Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-99342] - Immunochemical staining of human E6AP/UBE3A in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP2-99342] - Immunochemical staining of human E6AP/UBE3A in human ovarian cancer with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP2-99342-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP2-99342] - Immunofluorescence staining of E6AP/UBE3A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99342 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99342_rabbit-polyclonal-e6ap-ube3a-antibody-235202511494024.png)
![Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NBP2-99356-img0003.jpg "Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-99356-img0002.jpg)
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with NBP2-99356 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-99356_rabbit-polyclonal-fih-1-hif-1an-antibody-23520252135149.png)
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0003.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human brain with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NBP2-98763] - Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98763 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98763_rabbit-polyclonal-dnmt1-antibody-23520252135111.png)
![Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-59164-img0001.jpg "Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: OCT4 Antibody [NBP2-59164] - ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the antibody against OCT4 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/OCT4-Antibody-Chromatin-Immunoprecipitation-NBP2-59164-img0003.jpg)
![ELISA: OCT4 Antibody [NBP2-59164] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against OCT4. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100,000.](http://images.novusbio.com/fullsize/OCT4-Antibody-ELISA-NBP2-59164-img0002.jpg)