| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26303-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54483-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54477-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72136 | |||
![Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129] - Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 ug per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 (R) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NBP2-19129-img0003.jpg "Western Blot: STAT3 [p Tyr705] Antibody [NBP2-19129] - Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-a treatment. Load: 35 ug per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 (R) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.")
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NBP2-19129] - Rabbit anti STAT3 pY705 Antibody at 40X in colon tissue at pH 9. Tissue: Human Colon.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Immunohistochemistry-Paraffin-NBP2-19129-img0001.jpg)
![Immunohistochemistry of Rabbit anti STAT3 [p Tyr705] Antibody. Tissue: Human Colon at 40X in colon tissue at pH 9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/nbp2-19129_rabbit-polyclonal-stat3-p-tyr705-antibody-235202318143127.jpg)
![Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody [NB500-239] - Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-239-img0002.jpg)
![Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate. All lanes: Src Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 61 KDa Observed band size: 60, 61 KDa](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202411472113.jpg "Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate. All lanes: Src Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 61 KDa Observed band size: 60, 61 KDa")
![Immunocytochemistry/Immunofluorescence: Src Antibody (21H5) [NBP3-26303] - Staining of MCF-7 cells with Src Antibody (21H5) at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-28220241312247.jpg)
![Flow Cytometry: Src Antibody (21H5) [NBP3-26303] - Overlay histogram showing SH-SY5Y cells stained with Src Antibody (21H5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26303_rabbit-src-mab-21h5-282202414183653.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (1F8) [NBP2-53398] - Formalin-fixed, paraffin-embedded human Colon stained with RB1 Antibody (1F8).](http://images.novusbio.com/fullsize/RB1-Antibody-1F8-Immunohistochemistry-Paraffin-NBP2-53398-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (1F8) [NBP2-53398] - Formalin-fixed, paraffin-embedded human Colon stained with RB1 Antibody (1F8).")
![Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) [NBP2-53399] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).](http://images.novusbio.com/fullsize/RB1-Antibody-SPM353-Immunohistochemistry-Paraffin-NBP2-53399-img0001.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) [NBP2-53399] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).")
![Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01] - TCF3 monoclonal antibody (M01), clone 5G2 Analysis of TCF3 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/TCF-3-E2A-Antibody-5G2-Western-Blot-H00006929-M01-img0004.jpg "Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01] - TCF3 monoclonal antibody (M01), clone 5G2 Analysis of TCF3 expression in Hela S3 NE.")
![Sandwich ELISA: TCF-3/E2A Antibody (5G2) [H00006929-M01] - Detection limit for recombinant GST tagged TCF3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TCF-3-E2A-Antibody-5G2-Sandwich-ELISA-H00006929-M01-img0003.jpg)
![Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CLOCK-Antibody-HL1099-Western-Blot-NBP3-13718-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-910202420115014.jpg)
![Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-91020242041282.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-1610202419405734.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518395418.jpg "<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>mtTFA antibody [HL2012] detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.mtTFA stained by mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-immunohistochemistry-paraffin-141120251839548.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody [HL2012] (NBP3-48669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48669_rabbit-mttfa-mab-hl2012-western-blot-1411202518322913.jpg)
![Immunohistochemistry;YB1 Antibody[NBP3-29773]Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29785_rabbit-yb1-pab-78202410153552.jpg "Immunohistochemistry;YB1 Antibody[NBP3-29773]Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;YB1 Antibody[NBP3-29785]Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-YB1 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29785_rabbit-yb1-pab-78202410372636.jpg)
![Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Untreated (-) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-2120249374962.jpg "<b>Biological Strategies Validation. </b>Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Untreated (-) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-212024941332.jpg)
![Immunocytochemistry/Immunofluorescence: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - SNAI1 antibody [HL2303] detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAI1 stained by SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25689_rabbit-snail-mab-hl2303-azide-and-bsa-free-2120249425477.jpg)
![Western Blot: PKC zeta [p Thr410] Antibody [NBP3-13313] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta (phospho Thr410) antibody (NBP3-13313) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKC-zeta-[p-Thr410]-Antibody-Western-Blot-NBP3-13313-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKC zeta [p Thr410] Antibody [NBP3-13313] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta (phospho Thr410) antibody (NBP3-13313) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: PKC zeta [p Thr410] Antibody [NBP3-13313] - PKC zeta antibody detects PKC zeta protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.PKC zeta stained by PKC zeta antibody (NBP3-13313) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13313_rabbit-pkc-zeta-p-thr-410-pab-91020242091457.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC zeta [p Thr410] Antibody [NBP3-13313] - PKC zeta antibody detects PKC zeta protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKC zeta stained by PKC zeta antibody (NBP3-13313) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13313_rabbit-pkc-zeta-p-thr-410-pab-9102024201144.jpg)
![Immunohistochemistry-Paraffin: PKC zeta [p Thr410] Antibody [NBP3-13313] - PKC zeta antibody detects PKC zeta protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.PKC zeta stained by PKC zeta antibody (NBP3-13313) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13313_rabbit-pkc-zeta-p-thr-410-pab-91020242014682.jpg)
![Western Blot: HIF-3 alpha Antibody (OTI2D2) [NBP2-45735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A.](http://images.novusbio.com/fullsize/HIF-3-alpha-Antibody-2D2-Western-Blot-NBP2-45735-img0002.jpg "Western Blot: HIF-3 alpha Antibody (OTI2D2) [NBP2-45735] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A.")
![Immunohistochemistry: HIF-3 alpha Antibody (OTI2D2) [NBP2-45735] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HIF-3-alpha-Antibody-2D2-Immunohistochemistry-NBP2-45735-img0001.jpg)