| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06045-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00354-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99063-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98923-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05965-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97557-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06445-100ul | |||
![Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-278202415295515.jpg "Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32209) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29820241435282.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-2920241192373.jpg)
![Flow Cytometry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Flow cytometric analysis of HepG2 cells labeling Complement C5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29202416372127.jpg)
![Western Blot: BMP-4 Antibody (JE42-44) [NBP3-32086] - Western blot analysis of BMP-4 on different lysates with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32086) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-278202415295555.jpg "Western Blot: BMP-4 Antibody (JE42-44) [NBP3-32086] - Western blot analysis of BMP-4 on different lysates with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32086) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling BMP-4 with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32086, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29820241420987.jpg)
![Immunohistochemistry: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29820241420934.jpg)
![Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunocytochemistry analysis of HeLa cells labeling BMP-4 with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29202410484832.jpg)
![Western Blot: MRPS2 Antibody [NBP3-06045] - Anti-MRPS2 rabbit polyclonal antibody at 1:500 dilution Lane A: A-431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa](http://images.novusbio.com/fullsize/MRPS2-Antibody-Western-Blot-NBP3-06045-img0001.jpg "Western Blot: MRPS2 Antibody [NBP3-06045] - Anti-MRPS2 rabbit polyclonal antibody at 1:500 dilution Lane A: A-431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa")
![Immunohistochemistry-Paraffin: MRPS2 Antibody [NBP3-06045] - Immunochemical staining of human MRPS2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPS2-Antibody-Immunohistochemistry-Paraffin-NBP3-06045-img0003.jpg)
![Immunoprecipitation: MRPS2 Antibody [NBP3-06045] - MRPS2 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-MRPS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-MRPS2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa Observed band size :33 kDa](http://images.novusbio.com/fullsize/MRPS2-Antibody-Immunoprecipitation-NBP3-06045-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MRPS2 Antibody [NBP3-06045] - Immunofluorescence staining of MRPS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06045 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06045_rabbit-polyclonal-mrps2-antibody-235202514492343.png)
![Immunoprecipitation: COX4NB Antibody [NBP3-00354] - COX4NB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00354 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00354, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202511444480.png "Immunoprecipitation: COX4NB Antibody [NBP3-00354] - COX4NB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00354 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00354, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size: 24 kDa")
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human placenta with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202514492366.png)
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human pancreas with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202521451780.png)
![Western Blot: COX4NB Antibody [NBP3-00354] - NBP3-00354 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:24 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-235202514371320.png)
![Immunohistochemistry-Paraffin: COX4NB Antibody [NBP3-00354] - Immunochemical staining of human COX4NB in human skeletal muscle with NBP3-00354 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00354_rabbit-polyclonal-cox4nb-antibody-23520251628130.png)
![Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Western-Blot-NBP2-99063-img0003.jpg "Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-Immunohistochemistry-Paraffin-NBP2-99063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-99063 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202514331592.png)
![Immunoprecipitation: Calbindin D-28K Antibody [NBP2-99063] - Calbindin D-28K was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 ul NBP2-99063 and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: NBP2-99063, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp2-99063_rabbit-polyclonal-calbindin-d-28k-antibody-235202516335024.png)
![Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa](http://images.novusbio.com/fullsize/Actin-Antibody-Western-Blot-NBP2-98923-img0006.jpg "Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa")
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0005.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0004.jpg)
![Western Blot: NDUFB8 Antibody [NBP3-05965] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize/NDUFB8-Antibody-Western-Blot-NBP3-05965-img0001.jpg "Western Blot: NDUFB8 Antibody [NBP3-05965] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
![Immunohistochemistry-Paraffin: NDUFB8 Antibody [NBP3-05965] - Staining of human NDUFB8 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NDUFB8-Antibody-Immunohistochemistry-Paraffin-NBP3-05965-img0002.jpg)
![Immunoprecipitation: NDUFB8 Antibody [NBP3-05965] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NDUFB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFB8 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :22 kDa](http://images.novusbio.com/fullsize/NDUFB8-Antibody-Immunoprecipitation-NBP3-05965-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFB8 Antibody [NBP3-05965] - Staining of NDUFB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05965 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-05965_rabbit-polyclonal-ndufb8-antibody-235202521451721.png)
![Western Blot: G3BP1 Antibody (JE35-20) [NBP3-32358] - Western blot analysis of G3BP1 on different lysates with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: N2A cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32358) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-278202416285287.png "Western Blot: G3BP1 Antibody (JE35-20) [NBP3-32358] - Western blot analysis of G3BP1 on different lysates with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: N2A cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32358) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: G3BP1 Antibody (JE35-20) [NBP3-32358] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32358) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-298202414161887.jpg)
![Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (JE35-20) [NBP3-32358] - Immunocytochemistry analysis of LOVO cells labeling G3BP1 with Rabbit anti-G3BP11 antibody (NBP3-32358) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-29202410334082.jpg)
![Flow Cytometry: G3BP1 Antibody (JE35-20) [NBP3-32358] - Flow cytometric analysis of A549 cells labeling G3BP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32358, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-29202416453267.png)
![Western Blot: DR1 Antibody [NBP2-97557] - Anti-DR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/DR1-Antibody-Western-Blot-NBP2-97557-img0005.jpg "Western Blot: DR1 Antibody [NBP2-97557] - Anti-DR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-97557] - Immunochemical staining of human DR1 in human gallbladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/DR1-Antibody-Immunohistochemistry-Paraffin-NBP2-97557-img0004.jpg)
![Immunoprecipitation: DR1 Antibody [NBP2-97557] - DR1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-DR1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DR1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/DR1-Antibody-Immunoprecipitation-NBP2-97557-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DR1 Antibody [NBP2-97557] - Immunofluorescence staining of DR1 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97557 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97557_rabbit-polyclonal-dr1-antibody-235202514492361.png)
![Western Blot: NASP Antibody [NBP3-06445] - NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :180 kDa.](http://images.novusbio.com/fullsize/NASP-Antibody-Western-Blot-NBP3-06445-img0004.jpg "Western Blot: NASP Antibody [NBP3-06445] - NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :180 kDa.")
![Immunohistochemistry-Paraffin: NASP Antibody [NBP3-06445] - Immunochemical staining of human NASP in human rectum with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NASP-Antibody-Immunohistochemistry-Paraffin-NBP3-06445-img0002.jpg)
![Western Blot: NASP Antibody [NBP3-06445] - Anti-NASP rabbit polyclonal antibody at 1:2000 dilution. Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:180 kDa.](http://images.novusbio.com/fullsize/NASP-Antibody-Western-Blot-NBP3-06445-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NASP Antibody [NBP3-06445] - Immunofluorescence staining of NASP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06445 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06445_rabbit-polyclonal-nasp-antibody-23520251664715.png)
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Western-Blot-NBP2-59269-img0002.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Immunofluorescence-NBP2-59269-img0004.jpg)
![Chromatin Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - ChIP assays were performed using human HeLa cells, the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Chromatin-Immunoprecipitation-NBP2-59269-img0003.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Western-Blot-NBP2-59269-img0001.jpg)