| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97446-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90208-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06536-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97091-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98916-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00348-50ul | |||
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-EM-05-Flow-Cell-Surface-NBP1-44763-img0002.jpg "Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.")
![Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-288202412442931.jpg "Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29820241411541.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunocytochemistry analysis of HeLa cells labeling RNA Helicase A with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29202410484888.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Flow-Cytometry-NBP2-90208-img0003.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t")
![<b>Biological Strategies Validation. </b>Western Blot: ERp72 Antibody (020) [NBP2-90208] - Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Western-Blot-NBP2-90208-img0006.jpg)
![Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208] - PDIA4 was immunoprecipitated using: Lane A :0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Immunoprecipitation-NBP2-90208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] - Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90208 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90208_rabbit-monoclonal-erp72-antibody-020-235202511545653.png)
![Western Blot: IVD Antibody (04) [NBP3-06536] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa](http://images.novusbio.com/fullsize/IVD-Antibody-04-Western-Blot-NBP3-06536-img0004.jpg "Western Blot: IVD Antibody (04) [NBP3-06536] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IVD Antibody (04) [NBP3-06536] - Analysis of Human IVD expression on HeLa cells. The cells were treated, stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IVD-Antibody-04-Flow-Cytometry-NBP3-06536-img0002.jpg)
![Immunoprecipitation: IVD Antibody (04) [NBP3-06536] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IVD mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IVD mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa](http://images.novusbio.com/fullsize/IVD-Antibody-04-Immunoprecipitation-NBP3-06536-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: IVD Antibody (04) [NBP3-06536] - Staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-06536 antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06536_mouse-monoclonal-ivd-antibody-04-23520251664764.png)
![Western Blot: COX5b Antibody [NBP2-97091] - Anti-COX5b rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 13 kDa](http://images.novusbio.com/fullsize/COX5b-Antibody-Western-Blot-NBP2-97091-img0006.jpg "Western Blot: COX5b Antibody [NBP2-97091] - Anti-COX5b rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 13 kDa")
![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0004.jpg)
![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0003.jpg)
![Immunoprecipitation: COX5b Antibody [NBP2-97091] - COX5b was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-COX5b rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-COX5b rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/COX5b-Antibody-Immunoprecipitation-NBP2-97091-img0005.jpg)
![Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32376) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-278202415242668.jpg "Western Blot: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Western blot analysis of HABP1/C1QBP/GC1q R on different lysates with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Rat spleen tissue lysate (40 ug/Lane) Lane 9: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32376) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32376) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-298202414161836.jpg)
![Immunocytochemistry/ Immunofluorescence: HABP1/C1QBP/GC1q R Antibody (A8F4-R) [NBP3-32376] - Immunocytochemistry analysis of HeLa cells labeling HABP1/C1QBP/GC1q R with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HABP1/C1QBP/GC1q R antibody (NBP3-32376) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32376_mouse-habp1-c1qbp-gc1q-r-mab-a8f4-r-2920241114818.jpg)
![Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-278202415295516.jpg "Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-HADH antibody (NBP3-32416) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32416) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29820241420931.jpg)
![Immunocytochemistry/ Immunofluorescence: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunocytochemistry analysis of HeLa cells labeling HADH with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29202410334032.jpg)
![Western Blot: WDR77 Antibody [NBP2-98916] - Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/WDR77-Antibody-Western-Blot-NBP2-98916-img0002.jpg "Western Blot: WDR77 Antibody [NBP2-98916] - Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-98916] - Immunochemical staining of human WDR77 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP2-98916-img0004.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-98916] - Immunochemical staining of human WDR77 in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP2-98916-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP2-98916] - Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98916 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98916_rabbit-polyclonal-wdr77-antibody-235202511444432.png)
![Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-2352025162819.png "Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa")
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545641.png)
![Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-23520251664731.png)
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545651.png)