| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65351SS | |||
| Azide and BSA Free | NBP2-80648 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54385-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47993-0.1mg | |||
| IHC-Prediluted | NBP2-48110 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26371-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26439-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48336 | |||
| Azide and BSA Free | NBP3-11495 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50054SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45347 | |||
| Azide and BSA Free | NBP2-34420-0.1mg | |||
![Flow Cytometry: CD4 Antibody (OX-38) [NB100-65351] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-65351) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-OX-38-Flow-Cytometry-NB100-65351-img0001.jpg "Flow Cytometry: CD4 Antibody (OX-38) [NB100-65351] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-65351) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31/1395R).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Western-Blot-NBP2-53340-img0005.jpg "Western Blot: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31/1395R).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31/1395R).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Immunohistochemistry-Paraffin-NBP2-53340-img0003.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31/PECAM-1 Antibody (C31/1395R).followed by goat anti-rabbit- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Flow-Cytometry-NBP2-53340-img0002.jpg)
![Protein Array: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31/PECAM-1 Antibody (C31/1395R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Protein-Array-NBP2-53340-img0004.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Western-Blot-NBP2-44959-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6). Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Immunohistochemistry-Paraffin-NBP2-44959-img0001.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Western-Blot-NBP2-19397-img0002.jpg "Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19397-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] - MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242091420.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242010446.jpg)
![Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-262202414471544.jpg "Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-262202414471550.jpg)
![Western Blot: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Positive Western Blot detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: Peroxiredoxin 3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 26 kDa Observed band size: 20-28 kDa](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-282202411442649.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Staining of PC-3 cell with Peroxiredoxin 3 Antibody (14E2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 503-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-2822024131224.jpg)
![Flow Cytometry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Overlay Peak curve showing HepG2 cells stained with Peroxiredoxin 3 Antibody (14E2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-28220241431275.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Western Blot: BMPR-II Antibody [NBP1-32218] - Wild-type (WT) and BMPR2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMPR-II-Antibody-Western-Blot-NBP1-32218-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BMPR-II Antibody [NBP1-32218] - Wild-type (WT) and BMPR2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: BMPR-II Antibody [NBP1-32218] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMPR-II-Antibody-Western-Blot-NBP1-32218-img0002.jpg)
![Western Blot: Rabbit Polyclonal BMPR-II Antibody [NBP1-32218] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. BMP-2, NBP1-32218, was used at a dilution of 1:1000 and RUNX/CBFA1, NBP2-24755 was used at a dilution of 1:1000 in TBS Tween and 5% powdered milk. The protein was labeled at 150 kDa. Quantification of BMP-2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-32218_rabbit-polyclonal-bmpr-ii-antibody-western-blot-2952025142537..jpg)
![Western Blot: DPP3 Antibody [NBP1-31476] - DPP3 antibody [N2C1], Internal detects DPP3 protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. 7.5 % SDS-PAGE. DPP3 antibody [N2C1], Internal dilution: 1:1000](http://images.novusbio.com/fullsize/DPP3-Antibody-Western-Blot-NBP1-31476-img0006.jpg "Western Blot: DPP3 Antibody [NBP1-31476] - DPP3 antibody [N2C1], Internal detects DPP3 protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. 7.5 % SDS-PAGE. DPP3 antibody [N2C1], Internal dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: DPP3 Antibody [NBP1-31476] - Analysis of HeLa, using NBP1-31476 at 1:200 dilution.](http://images.novusbio.com/fullsize/DPP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31476-img0003.jpg)
![Western Blot: DPP3 Antibody [NBP1-31476] - Sample(30 ug of whole cell lysate) A:MOLT4 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DPP3-Antibody-Western-Blot-NBP1-31476-img0005.jpg)
![Immunohistochemistry: DPP3 Antibody [NBP1-31476] - DPP3 antibody [N2C1], Internal detects DPP3 protein at cytoplasm on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. DPP3 antibody [N2C1], Internal (NBP1-31476) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-31476_rabbit-polyclonal-dpp3-antibody-910202419593581.jpg)
![Immunocytochemistry/Immunofluorescence: CD20 Antibody (SPM618) [NBP2-44746] - Immunofluorescence staining of MOLT-4 cells using CD20 Antibody (SPM618) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD20-Antibody-SPM618-Immunocytochemistry-Immunofluorescence-NBP2-44746-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD20 Antibody (SPM618) [NBP2-44746] - Immunofluorescence staining of MOLT-4 cells using CD20 Antibody (SPM618) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: CD20 Antibody (SPM618) [NBP2-44746] - Human Tonsil stained with CD20 Monoclonal Antibody (SPM618)](http://images.novusbio.com/fullsize/CD20-Antibody-SPM618-Immunohistochemistry-Paraffin-NBP2-44746-img0001.jpg)
![Flow Cytometry: CD20 Antibody (SPM618) [NBP2-44746] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM618) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-SPM618-Flow-Cytometry-NBP2-44746-img0002.jpg)
![Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Western-Blot-NBP2-50054-img0004.jpg "Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0003.jpg)
![Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 antibody (SPM544).](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Western-Blot-NBP2-34373-img0003.jpg "Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 antibody (SPM544).")
![Immunohistochemistry-Paraffin: CD44 Antibody (SPM544) [NBP2-34373] - Formalin-fixed paraffin-embedded human tonsil stained with CD44 Monoclonal antibody (SPM544)](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Immunohistochemistry-Paraffin-NBP2-34373-img0001.jpg)
![Western Blot: CD44 Antibody (SPM544) [NBP2-34373] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (SPM544).](http://images.novusbio.com/fullsize/CD44-Antibody-SPM544-Western-Blot-NBP2-34373-img0002.jpg)