| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02952-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35163-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94173-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35077-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56140SS | |||
![Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2-Antibody-Western-Blot-NBP3-02952-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2 Antibody-Western Blot-NBP3-02952-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519302962.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519294585.jpg)
![Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Western-Blot-NBP3-16268-img0006.jpg "Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Mouse spinal cord using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Rat brain using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of NIH/3T3 cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-5220251265370.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of U-2 OS cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-522025125414.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411353099.jpg "Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Western blot analysis of various lysates using Protein Phosphatase 1 beta Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411364695.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunofluorescence analysis of U2OS cells using Protein Phosphatase 1 beta Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411392924.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411353020.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Human liver using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411364636.jpg)
![Western Blot: SKIP Antibody [NBP2-94173] - Analysis of extracts of Mouse skeletal muscle, using INPP5K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/SKIP-Antibody-Western-Blot-NBP2-94173-img0003.jpg "Western Blot: SKIP Antibody [NBP2-94173] - Analysis of extracts of Mouse skeletal muscle, using INPP5K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: SKIP Antibody [NBP2-94173] - Analysis of PC-12 cells using INPP5K Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SKIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94173-img0007.jpg)
![Western Blot: SKIP Antibody [NBP2-94173] - Analysis of extracts of 293T cells, using INPP5K antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SKIP-Antibody-Western-Blot-NBP2-94173-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of HeLa cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94173_rabbit-polyclonal-skip-antibody-52202519313477.jpg)
![Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of A-549 cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94173_rabbit-polyclonal-skip-antibody-5220251931340.jpg)
![Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of NIH/3T3 cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94173_rabbit-polyclonal-skip-antibody-5220251931338.jpg)
![Immunoprecipitation: Myosin Phosphatase Antibody [NBP3-35077] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Myosin Phosphatase antibody. Western blot was performed from the immunoprecipitate using Myosin Phosphatase antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415161825.jpg "Immunoprecipitation: Myosin Phosphatase Antibody [NBP3-35077] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Myosin Phosphatase antibody. Western blot was performed from the immunoprecipitate using Myosin Phosphatase antibody at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of HeLa cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415152878.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of PC-12 cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415175270.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of NIH/3T3 cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-91220241581217.jpg)
![Western Blot: Myosin Phosphatase Antibody [NBP3-35077] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415133450.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP3-35077] - Immunofluorescence analysis of U2OS cells using Myosin Phosphatase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35077_rabbit-myosin-phosphatase-pab-912202415111826.jpg)
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0005.jpg "Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of various cell lines, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Knockout-Validated-NBP3-15666-img0001.jpg)
![Immunoprecipitation: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug PTEN antibody (NBP3-15666). Western blot was performed from the immunoprecipitate using PTEN antibody (NBP3-15666) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunoprecipitation-NBP3-15666-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN Antibody (7O6F4) [PTEN] - Confocal imaging of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15666_rabbit-pten-mab-7o6f4-52202517361229.jpg)
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Western-Blot-NB110-85534-img0006.jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.")
![ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-ELISA-NB110-85534-img0007.jpg)
![Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.](http://images.novusbio.com/fullsize/nb110-85534_rabbit-polyclonal-fibroblast-activation-protein-alpha-fap-antibody-71020241239278.png)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)
![Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56140] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:1000 conc. of PTPN13 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PTPN13 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/PTPN13-PTPL1-Antibody-Immunohistochemistry-Paraffin-NB100-56140-img0001.jpg "Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56140] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:1000 conc. of PTPN13 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PTPN13 was observed. Staining was performed by Histowiz.")
![Immunohistochemistry-Paraffin: MTMR9 Antibody [NB100-832] - Staining of paraffin embedded Rat Spleen. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.](http://images.novusbio.com/fullsize/MTMR9-Antibody-Immunohistochemistry-Paraffin-NB100-832-img0001.jpg "Immunohistochemistry-Paraffin: MTMR9 Antibody [NB100-832] - Staining of paraffin embedded Rat Spleen. Steamed antigen retrieval with citrate buffer pH 6, DAB-staining.")
![Immunocytochemistry/Immunofluorescence: MTMR9 Antibody [NB100-832] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-832_goat-polyclonal-mtmr9-antibody-131120231145589.jpg)
![Immunocytochemistry/Immunofluorescence: MTMR9 Antibody [NB100-832] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-832_goat-polyclonal-mtmr9-antibody-131120231145581.jpg)
![Flow Cytometry: MTMR9 Antibody [NB100-832] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-832_goat-polyclonal-mtmr9-antibody-131120231145582.jpg)
![Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NB110-38889-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0010.jpg "Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunocytochemistry-Immunofluorescence-H00000053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunohistochemistry-Paraffin-H00000053-M01-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi . Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0004.jpg)
![ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01] - Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-ELISA-H00000053-M01-img0009.jpg)