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![Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776] - Western blot of mouse mitochondria from whole brain lysateshowing specific immunolabeling of the ~46 kDa CK1Mt proteinphosphorylated at Tyr153 in the first lane (-). Phosphospecificityis shown in the second lane (+) where the immunolabeling isgreatly decreased by blot treatment with lambda phosphatase(Lambda-Ptase, 1200 units for 30 minutes).](http://images.novusbio.com/fullsize/Creatine-kinase-MT-1A-[p-Tyr153]-Antibody-Western-Blot-NBP2-60776-img0001.jpg "Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776] - Western blot of mouse mitochondria from whole brain lysateshowing specific immunolabeling of the ~46 kDa CK1Mt proteinphosphorylated at Tyr153 in the first lane (-). Phosphospecificityis shown in the second lane (+) where the immunolabeling isgreatly decreased by blot treatment with lambda phosphatase(Lambda-Ptase, 1200 units for 30 minutes).")
![Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Human brain tissue lysate (40 ug/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33070_rabbit-dtk-tyro3-mab-je38-13-288202414191540.jpg "Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Human brain tissue lysate (40 ug/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody - BSA Free [NB110-55539] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Insulin R-CD220 Antibody - BSA Free-Immunohistochemistry-Paraffin-NB110-55539-img0003.jpg "Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody - BSA Free [NB110-55539] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr153-Antibody-EM-17-Western-Blot-NBP2-62192-img0001.jpg "Western Blot: CD3 zeta [p Tyr153] Antibody (EM-17) [NBP2-62192] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.")
![Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814] - Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (NBP3-32814) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: MCF7 treated with pp for 1 hour whole cell lysate Lane 4: MCF7 treated with 0.5uM Oligomycin for 30 minutes, then treated with pp for 1 hour whole cell lysate Lysates/proteins at 14 ug/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32814) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32814_rabbit-ulk1-p-ser556-mab-je59-28-288202412461674.jpg "Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814] - Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (NBP3-32814) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: MCF7 treated with pp for 1 hour whole cell lysate Lane 4: MCF7 treated with 0.5uM Oligomycin for 30 minutes, then treated with pp for 1 hour whole cell lysate Lysates/proteins at 14 ug/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32814) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PACSIN3 Antibody [NBP2-19673] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PACSIN3-Antibody-Western-Blot-NBP2-19673-img0001.jpg "Western Blot: PACSIN3 Antibody [NBP2-19673] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: MAPKAPK2 Antibody (4G7) [NBP3-15172] - Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, HL-60 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 50 KDa.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-4G7-Western-Blot-NBP3-15172-img0001.jpg "Western Blot: MAPKAPK2 Antibody (4G7) [NBP3-15172] - Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, HL-60 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 50 KDa.")
![Western Blot: MAP3K12 binding inhibitory protein 1 Antibody [NBP2-17219] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MAP3K12-binding-inhibitory-protein-1-Antibody-Western-Blot-NBP2-17219-img0001.jpg "Western Blot: MAP3K12 binding inhibitory protein 1 Antibody [NBP2-17219] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PRAK Antibody [NBP1-33093] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRAK-Antibody-Western-Blot-NBP1-33093-img0002.jpg "Western Blot: PRAK Antibody [NBP1-33093] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: PKC theta Antibody (1B2) [NBP3-15185] - Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, Mouse Brain whole cell lysate. All lanes: PKC theta antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82, 75, 68 KDa. Observed band size: 82, 55 KDa.](http://images.novusbio.com/fullsize/PKC-theta-Antibody-1B2-Western-Blot-NBP3-15185-img0001.jpg "Western Blot: PKC theta Antibody (1B2) [NBP3-15185] - Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, Mouse Brain whole cell lysate. All lanes: PKC theta antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82, 75, 68 KDa. Observed band size: 82, 55 KDa.")
![Western Blot: Fructosamine-3-kinase-related Antibody [NBP2-16536] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fructosamine-3-kinase-related-Antibody-Western-Blot-NBP2-16536-img0001.jpg "Western Blot: Fructosamine-3-kinase-related Antibody [NBP2-16536] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.")