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![Western Blot: PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39) [NBP3-32819] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32819) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32819) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32819_rabbit-pi-3-kinase-p85-alpha-p-tyr467-p-tyr199-mab-288202412442983.jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39) [NBP3-32819] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32819) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32819) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: EphA4 Antibody (M280) [NBP3-23328] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23328_mouse-epha4-mab-m280-1992023185697.jpg "Western Blot: EphA4 Antibody (M280) [NBP3-23328] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).")
![Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23232_rabbit-p38-alpha-pab-199202312403713.jpg "Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Western Blot: NEK11 Antibody [NBP2-19509] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/NEK11-Antibody-Western-Blot-NBP2-19509-img0001.jpg "Western Blot: NEK11 Antibody [NBP2-19509] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: NRBP2 Antibody [NBP2-19585] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NRBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-19585-img0002.jpg "Immunohistochemistry-Paraffin: NRBP2 Antibody [NBP2-19585] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.")
![Western Blot: IPPK Antibody [NBP2-16985] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/IPPK-Antibody-Western-Blot-NBP2-16985-img0001.jpg "Western Blot: IPPK Antibody [NBP2-16985] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: CLK2 Antibody [NBP1-32647] - (30 ug whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/CLK2-Antibody-Western-Blot-NBP1-32647-img0001.jpg "Western Blot: CLK2 Antibody [NBP1-32647] - (30 ug whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:1000")
![Western Blot: PHKG2 Antibody [NBP2-19802] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PHKG2-Antibody-Western-Blot-NBP2-19802-img0001.jpg "Western Blot: PHKG2 Antibody [NBP2-19802] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Thr2609]-Antibody-Western-Blot-NB600-897-img0005.jpg "Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.")
![Western Blot results of Rabbit DNA-PKcs [p Thr2609] Antibody. Lane 1: Molecular Weight Ladder. Lane 2: DNA Pkc phosphor control. Lane 3: DNA Pkc control. Lane 4: BSA. Load: 10ug. Primary Antibody: Rabbit Anti-DNA Pkcs pT609 at 1ug/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP](http://images.novusbio.com/fullsize/nb600-897_rabbit-polyclonal-dna-pkcs-p-thr2609-antibody-25520231328193.jpg)
![Western Blot: GRK6 Antibody [NBP2-32197] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GRK6 antibody NBP2-32197 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRK6-Antibody-Western-Blot-NBP2-32197-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: GRK6 Antibody [NBP2-32197] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GRK6 antibody NBP2-32197 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: Casein Kinase 1 delta Antibody (C1) [NBP3-41931] - DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 10ug/ml NBP3-41931; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41931_mouse-casein-kinase-1-delta-mab-c1-61220242195775.jpg "Immunohistochemistry-Paraffin: Casein Kinase 1 delta Antibody (C1) [NBP3-41931] - DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 10ug/ml NBP3-41931; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Casein Kinase 1 delta Antibody (C1) [NBP3-41931] - DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml NBP3-41931; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41931_mouse-casein-kinase-1-delta-mab-c1-612202421373068.jpg)