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![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Immunohistochemistry-Paraffin: EphA4 Antibody - BSA Free [NB110-39005] - Analysis of anti-EPHA4 antibody with human glomerulus at concentration 18 ug/ml.](http://images.novusbio.com/fullsize/EphA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-39005-img0003.jpg "Immunohistochemistry-Paraffin: EphA4 Antibody - BSA Free [NB110-39005] - Analysis of anti-EPHA4 antibody with human glomerulus at concentration 18 ug/ml.")
![Western Blot: Yes Antibody (JE37-26) [NBP3-33107] - Western blot analysis of Yes on different lysates with Rabbit anti-Yes antibody (NBP3-33107) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33107) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33107_rabbit-yes-mab-je37-26-28820241419155.jpg "Western Blot: Yes Antibody (JE37-26) [NBP3-33107] - Western blot analysis of Yes on different lysates with Rabbit anti-Yes antibody (NBP3-33107) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33107) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Yes Antibody (JE37-26) [NBP3-33107] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Yes antibody (NBP3-33107) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33107) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33107_rabbit-yes-mab-je37-26-298202414304176.jpg)
![Western Blot: MELK Antibody (PSH03-55) [NBP3-32587] - Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (NBP3-32587) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32587) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32587_rabbit-melk-mab-psh03-55-27820241633126.jpg "Western Blot: MELK Antibody (PSH03-55) [NBP3-32587] - Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (NBP3-32587) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32587) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32587_rabbit-melk-mab-psh03-55-298202413585532.jpg)
![Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela](http://images.novusbio.com/fullsize/MLKL-Antibody-SA40-04-Western-Blot-NBP2-67299-img0002.jpg "Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela")
![Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MLKL-Antibody-SA40-04-Immunohistochemistry-Paraffin-NBP2-67299-img0001.jpg)
![Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-288202412442996.jpg "Western Blot: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32818) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha [p Tyr199, p Tyr467] Antibody (PSH01-38) [NBP3-32818] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling PI 3-Kinase p85 alpha with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32818) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32818_rabbit-pi-3-kinase-p85-alpha-p-tyr199-p-tyr467-mab-29202410334087.jpg)
![Western Blot: EphA4 [p Tyr779] Antibody [NBP3-23330] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23330_rabbit-epha4-p-tyr779-pab-11920231665310.jpg "Western Blot: EphA4 [p Tyr779] Antibody [NBP3-23330] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).")
![Western Blot: EphA4 [p Tyr602] Antibody [NBP3-23329] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23329_rabbit-epha4-p-tyr602-pab-199202312403721.jpg "Western Blot: EphA4 [p Tyr602] Antibody [NBP3-23329] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).")
![Immunohistochemistry-Paraffin: DCLK1 Antibody (C8) [NBP3-41776] - DAB staining on IHC-P; Samples: Human Cerebellum Tissue; Primary Ab: 20ug/ml NBP3-41776; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41776_mouse-dclk1-mab-c8-61220242116232.jpg "Immunohistochemistry-Paraffin: DCLK1 Antibody (C8) [NBP3-41776] - DAB staining on IHC-P; Samples: Human Cerebellum Tissue; Primary Ab: 20ug/ml NBP3-41776; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: DCLK1 Antibody (C8) [NBP3-41776] - DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 20ug/ml NBP3-41776; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41776_mouse-dclk1-mab-c8-61220242058883.jpg)
![Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Western-Blot-NBP2-43733-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].")
![<b>Biological Strategies Validation. </b>Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (NBP2-43733) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43733_mouse-monoclonal-hist1h3a-ac-lys9-antibody-464-immunocytochemistry-immunofluorescence-2611202518413911.jpg)
![Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP2-17273] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MEKK3-Antibody-Immunohistochemistry-Paraffin-NBP2-17273-img0002.jpg "Immunohistochemistry-Paraffin: MEKK3 Antibody [NBP2-17273] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.")
![MEKK3 antibody [N3C2], Internal detects MEKK3 protein by western blot analysis.A. 30 ug 293T whole cell extractB. 30 ug whole cell extract of 3xFlag-human MAP3K3-transfected 293T cells7.5 % SDS-PAGEMEKK3 antibody [N3C2], Internal (NBP2-17273) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-17273_rabbit-polyclonal-mekk3-antibody-western-blot-2611202518294917.jpg)
![Immunohistochemistry-Paraffin: Protein kinase-like protein SgK493 Antibody [NBP2-19960] - H358 xenograft, using SGK493 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Protein-kinase-like-protein-SgK493-Antibody-Immunohistochemistry-Paraffin-NBP2-19960-img0002.jpg "Immunohistochemistry-Paraffin: Protein kinase-like protein SgK493 Antibody [NBP2-19960] - H358 xenograft, using SGK493 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Protein kinase-like protein SgK493 Antibody [NBP2-19960] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGK493 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19960_rabbit-polyclonal-protein-kinase-like-protein-sgk493-antibody-1610202419405765.jpg)