| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26247-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26158-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26486-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
![Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-199202312523619.jpg "Western Blot: Neuropilin-1 Antibody [NBP3-23182] - Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NBP3-23182) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody [NBP3-23182] - Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NBP3-23182) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide in the presence of the anti-NRP-1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23182_rabbit-neuropilin-1-pab-19920231856956.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)
![Western Blot: STAT2 [p Tyr690] Antibody [NB110-86975] - Analysis of Stat2pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000U/mL of IFN-a for 15 min at 37oC. Primary antibody was used at 1:1,000.](http://images.novusbio.com/fullsize/STAT2-[p-Tyr690]-Antibody-Western-Blot-NB110-86975-img0003.jpg "Western Blot: STAT2 [p Tyr690] Antibody [NB110-86975] - Analysis of Stat2pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000U/mL of IFN-a for 15 min at 37oC. Primary antibody was used at 1:1,000.")
![STAT2 [p Tyr690] Antibody showing nuclear positivity in epithelial cells of mouse urinary bladder tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-STAT2 pY690 antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/nb110-86975_rabbit-polyclonal-stat2-p-tyr690-antibody-23520231814582.jpg)
![Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-27220248313714.jpg "Immunohistochemistry: MAD2L2 Antibody (4D8) [NBP3-26158] - Image of MAD2L2 Antibody (4D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: MAD2L2 Antibody (4D8) [NBP3-26158] - Positive Western Blot detected in: 293 whole cell lysate. All lanes: MAD2L2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 kDa Observed band size: 25 kDa](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-28220241140925.jpg)
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (4D8) [NBP3-26158] - Staining of Hela Cells with MAD2L2 Antibody (4D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26158_rabbit-mad2l2-mab-4d8-282202412475128.jpg)
![Immunohistochemistry-Paraffin: Phospho-Tyrosine Antibody (PY20) [NBP2-29402] - Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.](http://images.novusbio.com/fullsize/Phospho-Tyrosine-Antibody-PY20-Immunohistochemistry-Paraffin-NBP2-29402-img0003.jpg "Immunohistochemistry-Paraffin: Phospho-Tyrosine Antibody (PY20) [NBP2-29402] - Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.")
![Western Blot: MEF2D Antibody [NBP1-80566] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80567 which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Western-Blot-NBP1-80566-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MEF2D Antibody [NBP1-80566] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80567 which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: MEF2D Antibody [NBP1-80566] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- MEF2D used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-NBP1-80566-img0004.jpg)
![Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-26220241322158.jpg "Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-262202415253321.jpg)
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-2722024827492.jpg)
![Western Blot: Aurora B Antibody (4E5) [NBP3-26486] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: Aurora B Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 36, 17, 35 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-282202411442635.jpg)
![Western Blot: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not). All lanes PKR antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa. Observed band size: 62 KDa.](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Western-Blot-NBP3-15204-img0003.jpg "Western Blot: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not). All lanes PKR antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa. Observed band size: 62 KDa.")
![Immunocytochemistry/Immunofluorescence: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Immunofluorescence staining of Hela cells (treated with 50mM Calyculin A for 30 minutes) with at PKR antibody 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Immunocytochemistry-Immunofluorescence-NBP3-15204-img0001.jpg)
![Immunohistochemistry: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - IHC image of PKR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/PKR-p-Thr449-Antibody-2F8-Immunohistochemistry-NBP3-15204-img0002.jpg)
![Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Western-Blot-NBP2-37703-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Chk1, Phospho (S317) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Immunohistochemistry-NBP2-37703-img0003.jpg)
![Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (50 ug) from HeLa cells treated with 500 uM EPE for 4 hours (+) or mock treated (-) cells Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody used for WB at 1 ug/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 500 uM EPE for 4 hours (+). Antibodies: Phospho SMC3 was immunoprecipitated (IP) using rabbit anti-Phospho SMC3 (S1083) antibody. The IP reactions were either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody was used for western blot (WB) of IPs at 1 ug/ml (upper panel); to detect total SMC3 affinity purified rabbit anti-SMC3 was used for WB of IPs at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Immunoprecipitation-NBP2-37694-img0004.jpg)
![SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 at 6 ug per reaction. Total SMC3 was immunoprecipitated with another rabbit anti-SMC3 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-NBP2-37694-img0005.jpg)
![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg)
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)