Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
PY20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free Summary

Immunogen
Phosphotyrosine was used as the immunogen for the Phosphotyrosine PY20 antibody.
Localization
Depends upon the location of phosphorylated target
Specificity
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • CyTOF-ready
Application Notes
Immunohistochemistry-Frozen, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Western Blot cited in literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Phospho-Tyrosine Antibody (PY20) - Azide and BSA Free

  • 2 amino 3(4 hydroxyphenyl) propanoic acid
  • 4 hydroxyphenylalanine
  • PhosphoTyrosine
  • pTyrosine
  • Tyrosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Phospho-Tyrosine Antibody (NBP2-33166) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Phospho-Tyrosine Products

Phospho-Tyrosine NBP2-33166

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