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![Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Western-Blot-NBP1-44893-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr1173-Antibody-EM-13-Immunoprecipitation-NBP1-44893-img0002.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Western-Blot-NBP2-59258-img0001.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - HeLa cells were stained with the antibody against H4K20me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Immunofluorescence-NBP2-59258-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - ChIP assays were performed using human HeLa cells, the antibody against H4K20me1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the GAPDH promoter and a region located 1 kb upstream of the GAPDH promoter, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59258-img0003.jpg)
![ELISA: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K20me1 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:51,100.](http://images.novusbio.com/fullsize/Histone-H4-[Methyl-Lys20]-Antibody-ELISA-NBP2-59258-img0002.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Western-Blot-NBP2-59246-img0001.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - HeLa cells were stained with the antibody against H4K20me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4K20me2 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Immunofluorescence-NBP2-59246-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 ug of antibody was used per ChIP experiment. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Chromatin-Immunoprecipitation-NBP2-59246-img0002.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - To test the cross reactivity of the antibody against H4K20me2, analysis was performed with peptides containing other histone modifications and the unmodified H4K20. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Dot-Blot-NBP2-59246-img0004.jpg)
![Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MAP4 was also immunoprecipitated by rabbit anti-MAP4 antibody NB100-74615, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/MAP4-Antibody-Western-Blot-NB100-74616-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MAP4 was also immunoprecipitated by rabbit anti-MAP4 antibody NB100-74615, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: MAP4 Antibody [NB100-74616] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAP4-Antibody-Immunohistochemistry-NB100-74616-img0005.jpg)
![Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-MAP4 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/MAP4-Antibody-Immunohistochemistry-Paraffin-NB100-74616-img0003.jpg)
![Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.](http://images.novusbio.com/fullsize/Smad3-[p-Thr179]-Antibody-Western-Blot-NBP2-19126-img0001.jpg "Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.")
![ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Beta signaling pathway. TGF- Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD.i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.](http://images.novusbio.com/fullsize/Smad3 [p Thr179] Antibody-ELISA-NBP2-19126-img0003.jpg)
![Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] - Image of FOXO3 Antibody (1E2) diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26571_rabbit-foxo3-mab-1e2-2722024850466.jpg "Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] - Image of FOXO3 Antibody (1E2) diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] - Image of FOXO3 Antibody (1E2) diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26571_rabbit-foxo3-mab-1e2-2722024847198.jpg)
![Western Blot: FOXO3 Antibody (1E2) [NBP3-26571] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate. All lanes: FOXO3 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 72, 49 KDa Observed band size: 72-90 KDa](http://images.novusbio.com/fullsize/nbp3-26571_rabbit-foxo3-mab-1e2-28220241136307.jpg)
![Immunocytochemistry/Immunofluorescence: FOXO3 Antibody (1E2) [NBP3-26571] - Staining of PC3 cells with FOXO3 Antibody (1E2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26571_rabbit-foxo3-mab-1e2-28220241312244.jpg)
![Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ABI1-Antibody-Immunoprecipitation-NB100-59845-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABI1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ABI1-Antibody-Immunohistochemistry-NB100-59845-img0004.jpg)
![Western Blot: MEF2A Antibody [NBP1-32268] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MEF2A-Antibody-Western-Blot-NBP1-32268-img0004.jpg "Western Blot: MEF2A Antibody [NBP1-32268] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MEF2A Antibody [NBP1-32268] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MEF2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32268-img0003.jpg)
![MEF2A antibody [N1N2], N-term detects MEF2A protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: MEF2A stained by MEF2A antibody [N1N2], N-term (NBP1-32268) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp1-32268_rabbit-polyclonal-mef2a-antibody-immunohistochemistry-1411202518405419.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] (NBP2-42860) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42860_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-282-immunocytochemistry-immunofluorescence-26112025198364.jpg)
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-262202414471526.jpg "Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-2722024827496.jpg)
![Western Blot: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Positive Western Blot detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: Ku80/XRCC5 Antibody at 1: 2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa Observed band size: 83 kDa](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-282202411442624.jpg)
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Staining of Hela Cells with Ku80/XRCC5 Antibody (5G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-28220241312241.jpg)
![Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-262202415475643.jpg "Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: ERK2 Antibody (10C7) [NBP3-26150] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: ERK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 37 kDa Observed band size: 42 kDa](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-2822024114091.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (10C7) [NBP3-26150] - Staining of Hela Cells with ERK2 Antibody (10C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-28220241249366.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Western-Blot-NBP2-42861-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Chromatin-Immunoprecipitation-NBP2-42861-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (NBP2-42861) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42861_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-1851-immunocytochemistry-immunofluorescence-261120251962714.jpg)