| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26248-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26150-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26379-50ul | |||
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-262202414471526.jpg "Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-2722024827496.jpg)
![Western Blot: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Positive Western Blot detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: Ku80/XRCC5 Antibody at 1: 2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa Observed band size: 83 kDa](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-282202411442624.jpg)
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Staining of Hela Cells with Ku80/XRCC5 Antibody (5G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-28220241312241.jpg)
![Western Blot: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Affinity Purified Glycogen Synthase [p Ser641] antibody.Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'.Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'.Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'.Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12uL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG at 1:5,000 dilution preceded color development using Amersham's substrate system. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/Glycogen-Synthase-[p-Ser641]-Antibody-Western-Blot-NB600-611-img0008.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Affinity Purified Glycogen Synthase [p Ser641] antibody.Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'.Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'.Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'.Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12uL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG at 1:5,000 dilution preceded color development using Amersham's substrate system. Other detection methods will yield similar results.")
![Immunohistochemistry: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Immunohistochemistry with Glycogen Synthase [p Ser641] antibody.Tissue: Human Prostate.Fixation: formalin-fixed, paraffin-embedded tissue.Antigen retrieval: heat-induced.Primary antibody: 5 ug/ml.Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Glycogen-Synthase-[p-Ser641]-Antibody-Immunohistochemistry-NB600-611-img0009.jpg)
![Signal Stimulation: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Diagram of glycogen synthase as a component of insulin signal transduction pathways.](http://images.novusbio.com/fullsize/Glycogen-Synthase-[p-Ser641]-Antibody-Signal-Stimulation-NB600-611-img0006.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] (NBP2-42860) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42860_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-282-immunocytochemistry-immunofluorescence-26112025198364.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202414471571.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202415475635.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 KDa Observed band size: 36 KDa](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202411442641.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202412493624.jpg)
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-262202414471555.jpg "Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-2722024847191.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Positive Western Blot detected in: MCF-7 whole cell lysate, K562 whole cell lysate. All lanes: Histone Deacetylase 2/HDAC2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56, 52 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-282202411442634.jpg)
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Staining of Hela Cells with Histone Deacetylase 2/HDAC2 Antibody (9H4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-2822024132366.jpg)
![Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.](http://images.novusbio.com/fullsize/Smad3-[p-Thr179]-Antibody-Western-Blot-NBP2-19126-img0001.jpg "Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.")
![ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Beta signaling pathway. TGF- Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD.i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.](http://images.novusbio.com/fullsize/Smad3 [p Thr179] Antibody-ELISA-NBP2-19126-img0003.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Western-Blot-NBP2-42861-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Chromatin-Immunoprecipitation-NBP2-42861-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (NBP2-42861) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42861_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-1851-immunocytochemistry-immunofluorescence-261120251962714.jpg)
![Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MAP4 was also immunoprecipitated by rabbit anti-MAP4 antibody NB100-74615, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/MAP4-Antibody-Western-Blot-NB100-74616-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MAP4 was also immunoprecipitated by rabbit anti-MAP4 antibody NB100-74615, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: MAP4 Antibody [NB100-74616] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAP4-Antibody-Immunohistochemistry-NB100-74616-img0005.jpg)
![Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-MAP4 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/MAP4-Antibody-Immunohistochemistry-Paraffin-NB100-74616-img0003.jpg)
![Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-262202415475643.jpg "Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: ERK2 Antibody (10C7) [NBP3-26150] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: ERK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 37 kDa Observed band size: 42 kDa](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-2822024114091.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (10C7) [NBP3-26150] - Staining of Hela Cells with ERK2 Antibody (10C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-28220241249366.jpg)
![Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] - Image of PKN2 Antibody (20A8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-2722024856311.jpg "Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] - Image of PKN2 Antibody (20A8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PKN2 Antibody (20A8) [NBP3-26379] - Positive Western Blot detected in: Hela whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate. All lanes: PKN2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 113, 117, 112 kDa Observed band size: 130 kDa](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-282202411442639.jpg)
![Immunocytochemistry/Immunofluorescence: PKN2 Antibody (20A8) [NBP3-26379] - Staining of MCF-7 cell with PKN2 Antibody (20A8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-28220241247510.jpg)
![Flow Cytometry: PKN2 Antibody (20A8) [NBP3-26379] - Overlay Peak curve showing MCF7 cells stained with PKN2 Antibody (20A8) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-282202414183661.jpg)
![Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ABI1-Antibody-Immunoprecipitation-NB100-59845-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABI1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ABI1-Antibody-Immunohistochemistry-NB100-59845-img0004.jpg)
![Western Blot: MEF2A Antibody [NBP1-32268] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MEF2A-Antibody-Western-Blot-NBP1-32268-img0004.jpg "Western Blot: MEF2A Antibody [NBP1-32268] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MEF2A Antibody [NBP1-32268] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MEF2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32268-img0003.jpg)
![MEF2A antibody [N1N2], N-term detects MEF2A protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: MEF2A stained by MEF2A antibody [N1N2], N-term (NBP1-32268) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp1-32268_rabbit-polyclonal-mef2a-antibody-immunohistochemistry-1411202518405419.jpg)