| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26222-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81898-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18553-100ul | |||
| BSA Free | NBP3-18553-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43675-100ul | |||
| BSA Free | NBP3-43675-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-07986-100ul | |||
| BSA Free | NBP3-07986-25ul | |||
![Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Detection of Human and Mouse eIF2-alpha on HeLa whole cell lysate using NB100-2249.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Western-Blot-NB100-2249-img0001.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Detection of Human and Mouse eIF2-alpha on HeLa whole cell lysate using NB100-2249.")
![Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF2alpha/EIF2S1 used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunohistochemistry-Paraffin-NB100-2249-img0003.jpg)
![Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-[p-Tyr449]-Antibody-Western-Blot-NB110-85537-img0005.jpg "Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.")
![Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-[p-Tyr449]-Antibody-Western-Blot-NB110-85537-img0006.jpg)
![Western Blot of Rabbit aalpha/CD127 [p Tyr449] Antibody antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/nb110-85537_rabbit-polyclonal-il-7r-alpha-cd127-p-tyr449-antibody-255202313135153.jpg)
![Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Image of HNF-4 alpha/NR2A1 Antibody (10A7) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.](http://images.novusbio.com/fullsize/nbp3-26222_rabbit-hnf-4-alpha-nr2a1-mab-10a7-262202415253319.jpg "Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Image of HNF-4 alpha/NR2A1 Antibody (10A7) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.")
![Western Blot: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Positive Western Blot detected in: HL-60 whole cell lysate, Rat Liver tissue lysate. All lanes: HNF-4 alpha/NR2A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52 kDa Observed band size: 52 kDa](http://images.novusbio.com/fullsize/nbp3-26222_rabbit-hnf-4-alpha-nr2a1-mab-10a7-282202411442644.jpg)
![Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Staining of MCF-7 with HNF-4 alpha/NR2A1 Antibody (10A7) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 526-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26222_rabbit-hnf-4-alpha-nr2a1-mab-10a7-282202412493613.jpg)
![Flow Cytometry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Overlay Peak curve showing HepG2 cells stained with HNF-4 alpha/NR2A1 Antibody (10A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26222_rabbit-hnf-4-alpha-nr2a1-mab-10a7-282202414312784.jpg)
![Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81898_rabbit-polyclonal-eif4e-p-ser209-antibody-10320252346451.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4E [p Ser209] Antibody - BSA Free [NB100-81898] - Immunofluorescence staining of methanol-fixed MCF cells using NB100-81898.](http://images.novusbio.com/fullsize/nb100-81898_rabbit-polyclonal-eif4e-p-ser209-antibody-10320252346452.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Immunocytochemistry-Immunofluorescence-H00004633-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of MYL2 transfected lysate using anti-MYL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYL2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Immunoprecipitation-H00004633-M01-img0006.jpg)
![Sandwich ELISA: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Detection limit for recombinant GST tagged MYL2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Myosin-Light-Chain-2-Antibody-3B9-B4-Sandwich-ELISA-H00004633-M01-img0007.jpg)
![Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Western-Blot-NBP3-13072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0002.jpg)
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0004.jpg "Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Immunocytochemistry-Immunofluorescence-NBP3-13623-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0001.jpg)
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0003.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Immunocytochemistry-Immunofluorescence-NBP3-13617-img0002.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0003.jpg)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13617_mouse-hsp27-p-ser-82-mab-gt357-western-blot-2611202518484816.jpg)
![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0003.jpg "Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Untreated (-) and treated (+) U87-MG whole cell extracts (40 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0001.jpg)
![Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653--p-Tyr654]-Antibody-Western-Blot-NBP3-13251-img0002.jpg)