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![Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13140] - Untreated (-) and treated (+) Jurkat whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vav-1 Tyr 174 antibody (NBP3-13140) diluted at 1:1000.](http://images.novusbio.com/fullsize/Vav-1-[p-Tyr174]-Antibody-Western-Blot-NBP3-13140-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13140] - Untreated (-) and treated (+) Jurkat whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vav-1 Tyr 174 antibody (NBP3-13140) diluted at 1:1000.")
![Western Blot: beta-Catenin [p Ser37, p Thr41, p Ser33] Antibody [NBP3-13118] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin (phospho Ser33/Ser37/Thr41) antibody (NBP3-13118) diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser37--p-Thr41--p-Ser33]-Antibody-Western-Blot-NBP3-13118-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser37, p Thr41, p Ser33] Antibody [NBP3-13118] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin (phospho Ser33/Ser37/Thr41) antibody (NBP3-13118) diluted at 1:500.")
![Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13139] - Untreated (-) and treated (+) Jurkat whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vav-1 Tyr 174 antibody (NBP3-13139) diluted at 1:1000.](http://images.novusbio.com/fullsize/Vav-1-[p-Tyr174]-Antibody-Western-Blot-NBP3-13139-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13139] - Untreated (-) and treated (+) Jurkat whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vav-1 Tyr 174 antibody (NBP3-13139) diluted at 1:1000.")
![Immunoprecipitation:PPAR gamma/NR1C3 AntibodyNBP3-30113] -Detection of mouse PPAR-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from differentiated mouse NIH 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody NBP3-30113 (lot NBP3-30113-1) used for IP at 6 µg per reaction. PPAR-gamma was also immunoprecipitated by rabbit anti-PPAR-gamma.. For blotting immunoprecipitated PPAR-gamma, NBP3-30113 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30113_rabbit-ppar-gamma-nr1c3-pab-301220241945452.jpg "Immunoprecipitation:PPAR gamma/NR1C3 AntibodyNBP3-30113] -Detection of mouse PPAR-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from differentiated mouse NIH 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody NBP3-30113 (lot NBP3-30113-1) used for IP at 6 µg per reaction. PPAR-gamma was also immunoprecipitated by rabbit anti-PPAR-gamma.. For blotting immunoprecipitated PPAR-gamma, NBP3-30113 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad2-[p-Ser255--p-Ser245--p-Ser250]-Antibody-Western-Blot-NBP3-13246-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: RNMT Overexpression Lysate (Adult Normal) [NBL1-15467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNMT.](http://images.novusbio.com/fullsize/RNMT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15467-img0002.jpg "Western Blot: RNMT Overexpression Lysate (Adult Normal) [NBL1-15467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNMT.")
![Western Blot: IRS1 [p Tyr1179] Antibody [NB100-74450] - Lysate from insulin-treated 3T3 L1 cells.](http://images.novusbio.com/fullsize/IRS1-[p-Tyr1179]-Antibody-Western-Blot-NB100-74450-img0003.jpg "Western Blot: IRS1 [p Tyr1179] Antibody [NB100-74450] - Lysate from insulin-treated 3T3 L1 cells.")
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP2-37834] - Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-37834_rabbit-polyclonal-nephrin-antibody-2711202419451236.jpg "Immunohistochemistry: Nephrin Antibody - BSA Free [NBP2-37834] - Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 ug/mL.")
![Western Blot: LBP Antibody [NBP1-58973] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/LBP-Antibody-Western-Blot-NBP1-58973-img0002.jpg "Western Blot: LBP Antibody [NBP1-58973] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Band 3 Antibody [NBP1-70433] - Human Liver cell lysate, concentration 0.1ug/ml.](http://images.novusbio.com/fullsize/Band-3-Antibody-Western-Blot-NBP1-70433-img0002.jpg "Western Blot: Band 3 Antibody [NBP1-70433] - Human Liver cell lysate, concentration 0.1ug/ml.")