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![Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr950]-Antibody-Western-Blot-NBP3-13235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Ser1046--p-Ser1047]-Antibody-Western-Blot-NBP3-13262-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Ser1046, p Ser1047] Antibody [NBP3-13262] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Ser1046/Ser1047) antibody (NBP3-13262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: PDGF R beta [p Tyr740] Antibody [NBP3-13269] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF R beta (phospho Tyr740) antibody (NBP3-13269) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PDGF-R-beta-[p-Tyr740]-Antibody-Western-Blot-NBP3-13269-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDGF R beta [p Tyr740] Antibody [NBP3-13269] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF R beta (phospho Tyr740) antibody (NBP3-13269) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: PDGF R beta [p Tyr771] Antibody [NBP3-13250] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF R beta (phospho Tyr771) antibody (NBP3-13250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PDGF-R-beta-[p-Tyr771]-Antibody-Western-Blot-NBP3-13250-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PDGF R beta [p Tyr771] Antibody [NBP3-13250] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF R beta (phospho Tyr771) antibody (NBP3-13250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: SCL/Tal1 Antibody (HL1703) - Azide and BSA Free [NBP3-25709] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [HL1703] (NBP3-25709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25709_rabbit-scl-tal1-mab-hl1703-azide-and-bsa-free-2120249374966.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SCL/Tal1 Antibody (HL1703) - Azide and BSA Free [NBP3-25709] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [HL1703] (NBP3-25709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 antibody [HL3583] (NBP3-48829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48829_rabbit-pka-r2-p-ser99-mab-hl3583-western-blot-141120251835468.jpg "Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 antibody [HL3583] (NBP3-48829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Chk2-[p-Ser19]-Antibody-Western-Blot-NBP3-13328-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Ser19] Antibody [NBP3-13328] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Ser19) antibody (NBP3-13328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: PLC-gamma 2 [p Tyr759] Antibody [NBP3-13241] - Untreated (-) and treated (+) Raji whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr759) antibody (NBP3-13241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLC-gamma-2-[p-Tyr759]-Antibody-Western-Blot-NBP3-13241-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLC-gamma 2 [p Tyr759] Antibody [NBP3-13241] - Untreated (-) and treated (+) Raji whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr759) antibody (NBP3-13241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Bcl-2 [p Ser70] Antibody [NBP3-12895] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 (phospho Ser70) antibody (NBP3-12895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bcl-2-[p-Ser70]-Antibody-Western-Blot-NBP3-12895-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Bcl-2 [p Ser70] Antibody [NBP3-12895] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 (phospho Ser70) antibody (NBP3-12895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")