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![Western Blot: Raf-1 Antibody [NBP3-23356] - Western blot of GST fusion protein containing human Raf-1. The blot was probed with polyclonal anti-Raf-1 (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of Raf-1 (C-terminus) blocking peptide (lane 2), Raf-1 (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).](http://images.novusbio.com/fullsize/nbp3-23356_rabbit-raf-1-pab-189202317355212.jpg "Western Blot: Raf-1 Antibody [NBP3-23356] - Western blot of GST fusion protein containing human Raf-1. The blot was probed with polyclonal anti-Raf-1 (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of Raf-1 (C-terminus) blocking peptide (lane 2), Raf-1 (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).")
![Western Blot: DOK1 [p Tyr362] Antibody [NBP3-23283] - Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23283_rabbit-dok1-p-tyr362-pab-19920231911471.jpg "Western Blot: DOK1 [p Tyr362] Antibody [NBP3-23283] - Western blot image of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr-362) (lanes 3 & 4), and anti-Dok1 (Ser-450) (lanes 5 & 6).")
![Western Blot: SHP-2/PTPN11 Antibody (M163) [NBP3-23400] - Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23400_mouse-shp-2-ptpn11-mab-m163-19920231410139.jpg "Western Blot: SHP-2/PTPN11 Antibody (M163) [NBP3-23400] - Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).")
![Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23359_rabbit-raf-1-p-ser471-pab-19920231915416.jpg "Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).")
![Western Blot: Raf-1 Antibody (M208) [NBP3-23358] - Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-Raf-1 (N-terminal) antibody at 1:500.](http://images.novusbio.com/fullsize/nbp3-23358_mouse-raf-1-mab-m208-19920231856927.jpg "Western Blot: Raf-1 Antibody (M208) [NBP3-23358] - Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-Raf-1 (N-terminal) antibody at 1:500.")
![Western Blot: Girdin [p Ser1416] Antibody [NBP3-23336] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1416), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23336_rabbit-girdin-p-ser1416-pab-19920231820298.jpg "Western Blot: Girdin [p Ser1416] Antibody [NBP3-23336] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1416), phospho-specific (lanes 3 & 4).")
![Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23435_rabbit-bad-p-ser112-pab-19920231410131.jpg "Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).")
![Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23372_rabbit-rhoa-p-ser188-pab-1992023141013.jpg "Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).")
![Western Blot: CRMP2 [p Thr555] Antibody (M539) [NBP3-23270] - Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23270_mouse-crmp2-p-thr555-mab-m539-19920231410129.jpg "Western Blot: CRMP2 [p Thr555] Antibody (M539) [NBP3-23270] - Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).")
![Western Blot: VAV3 Antibody [NBP3-23274] - Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-VAV2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-VAV3 (a.a. 293-305) at 1:500 (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23274_rabbit-vav3-pab-1992023191545.jpg "Western Blot: VAV3 Antibody [NBP3-23274] - Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-VAV2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-VAV3 (a.a. 293-305) at 1:500 (lanes 4-6).")
![Western Blot: N-WASP Antibody [NBP3-23279] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23279_rabbit-n-wasp-pab-1992023141016.jpg "Western Blot: N-WASP Antibody [NBP3-23279] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).")