| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16925-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35092-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38638-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38544-20ul | |||
![Western Blot: Paxillin [p Tyr118] Antibody (2E6P0) [NBP3-16929] - Western blot analysis of extracts of various cell lines, using Paxillin Rabbit mAb (NBP3-16929) at 1:1000 dilution.A-431 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Paxillin-p-Tyr118-Antibody-2E6P0-Western-Blot-NBP3-16929-img0001.jpg "Western Blot: Paxillin [p Tyr118] Antibody (2E6P0) [NBP3-16929] - Western blot analysis of extracts of various cell lines, using Paxillin Rabbit mAb (NBP3-16929) at 1:1000 dilution.A-431 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TBC1D4-[p-Ser588]-Antibody-Western-Blot-NBP3-05451-img0001.jpg "Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: eNOS [p Thr495] Antibody [NBP3-05410] - Western blot analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05410) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/eNOS [p Thr495] Antibody-Western Blot-NBP3-05410-img0001.jpg "Western Blot: eNOS [p Thr495] Antibody [NBP3-05410] - Western blot analysis of extracts of NIH/3T3 cells, using eNOS antibody (NBP3-05410) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cdk9-[p-Thr186]-Antibody-Western-Blot-NBP3-05455-img0001.jpg "Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/TSC2-[p-Ser1387]-Antibody-Western-Blot-NBP3-16925-img0001.jpg "Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: Cyclin H Antibody [NBP3-35092] - Western blot analysis of various lysates using Cyclin H Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35092_rabbit-cyclin-h-pab-1012202411592857.jpg "Western Blot: Cyclin H Antibody [NBP3-35092] - Western blot analysis of various lysates using Cyclin H Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Cyclin H Antibody [NBP3-35092] - Western Blot analysis of various lysates using Cyclin H Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35092_rabbit-cyclin-h-pab-31120251704773.jpg%3Ft=1706682198)
![Western Blot: Src [p Ser17] Antibody [NBP3-05433] - Western blot analysis of extracts of 293T cells, using Src antibody (NBP3-05433) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Src-[p-Ser17]-Antibody-Western-Blot-NBP3-05433-img0001.jpg "Western Blot: Src [p Ser17] Antibody [NBP3-05433] - Western blot analysis of extracts of 293T cells, using Src antibody (NBP3-05433) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NBP3-38638] - Western blot analysis of lysates from NIH/3T3 cells, using NFkB p105/p50 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38638_rabbit-nfkb-p105-p50-p-ser-337-pab-101220241154559.jpg "Western Blot: NFkB p105/p50 [p Ser337] Antibody [NBP3-38638] - Western blot analysis of lysates from NIH/3T3 cells, using NFkB p105/p50 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP3-38544] - Western blot analysis of various lysates using Fast skeletal myosin light chain 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38544_rabbit-fast-skeletal-myosin-light-chain-1-pab-912202414123852.jpg "Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP3-38544] - Western blot analysis of various lysates using Fast skeletal myosin light chain 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")