| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51582SS | |||
| Azide and BSA Free | NBP2-80720 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04965-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81798-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90269-25ul | |||
![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1 on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA.Exposure time: 1 minute 34 seconds](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0008.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1 on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA.Exposure time: 1 minute 34 seconds")
![Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunocytochemistry-Immunofluorescence-NBP2-67315-img0002.jpg)
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Glycogen synthase [p Ser641] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0010.jpg)
![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1(S641) on different lysates using anti-Phospho-Glycogen synthase 1(S641) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver lysate, untreated Lane 3: Mouse liver lysate, treated with AP](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0006.jpg)
![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Western blot analysis of Glycogen Synthase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. G](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunocytochemistry-Immunofluorescence-NBP2-67315-img0001.jpg)
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0003.jpg)
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0004.jpg)
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0005.jpg)
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1(S641) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0009.jpg)
![Western Blot: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Western-Blot-NBP2-67394-img0008.jpg "Western Blot: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain")
![Immunocytochemistry/Immunofluorescence: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunocytochemistry-Immunofluorescence-NBP2-67394-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Staining Phospho-Smad5(S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunocytochemistry-Immunofluorescence-NBP2-67394-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0006.jpg)
![Western Blot: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Western blot analysis of TrkB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Western-Blot-NBP2-67578-img0008.jpg "Western Blot: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Western blot analysis of TrkB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunocytochemistry-Immunofluorescence-NBP2-67578-img0007.jpg)
![Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded mouse pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunohistochemistry-Paraffin-NBP2-67578-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Staining phospho -TrkB(Y817) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunocytochemistry-Immunofluorescence-NBP2-67578-img0001.jpg)
![Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded human kidney tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunohistochemistry-Paraffin-NBP2-67578-img0002.jpg)
![Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded human pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunohistochemistry-Paraffin-NBP2-67578-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded mouse heart tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkB-p-Tyr817-Antibody-SC0556-Immunohistochemistry-Paraffin-NBP2-67578-img0004.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg)
![Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NB100-636-img0003.jpg)
![Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0002.jpg "Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.")
![<b>Biological Strategies Validation. </b>Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 incubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nbp1-04965_rabbit-polyclonal-eif4g1-p-ser1232-antibody-103202523464542.jpg)
![Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATF2-[p-Thr69]-Antibody-Western-Blot-NB100-81798-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ATF2 [p Thr69] Antibody [NB100-81798] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81798(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81798_rabbit-polyclonal-atf2-p-thr69-antibody-10320252332104.jpg)
![Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Analysis of extracts from 3T3 cells untreated or treated with Anisomycin using NB100-81798](http://images.novusbio.com/fullsize/nb100-81798_rabbit-polyclonal-atf2-p-thr69-antibody-10320252347260.jpg)
![Immunocytochemistry/Immunofluorescence: ATF2 [p Thr69] Antibody [NB100-81798] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81798](http://images.novusbio.com/fullsize/nb100-81798_rabbit-polyclonal-atf2-p-thr69-antibody-1132025155590.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90269 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90269 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0019.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human prostate shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ATP Citrate Lyase Antibody [NBP1-90269] - Analysis using Anti-ACLY antibody NBP1-90269 (A) shows similar pattern to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90269-img0010.jpg)
![Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-simple-western-3032023112047.jpg "Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Flow-Cytometry-NBP2-76399-img0007.jpg)
![Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-NBP2-76399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-Paraffin-NBP2-76399-img0009.jpg)
![Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunoprecipitation-NBP2-76399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry-paraffin-732024101321..jpg)
![Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-western-blot-732024101522..jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90266 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90266 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0019.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0017.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90266-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ATP Citrate Lyase Antibody [NBP1-90266] - Analysis using Anti-ACLY antibody NBP1-90266 (A) shows similar pattern to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90266-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89544-img0011.jpg "Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues . Corresponding JUNB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0016.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0013.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0014.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0012.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0015.jpg)
