| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26431-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26361-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26164-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26681-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26421-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26357-50ul | |||
![Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: ATF2 [p Thr71] Antibody at 1.015ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 KDa Observed band size: 70 KDa](http://images.novusbio.com/fullsize/nbp3-26431_rabbit-atf2-p-thr-71-mab-4f2-28220241152514.jpg "Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: ATF2 [p Thr71] Antibody at 1.015ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 KDa Observed band size: 70 KDa")
![Immunocytochemistry/Immunofluorescence: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Staining of A549 cells(treated with 100mM EGF for 20min) with ATF2 [p Thr71] Antibody (4F2) at 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26431_rabbit-atf2-p-thr-71-mab-4f2-28220241323615.jpg)
![Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.](http://images.novusbio.com/fullsize/SMC3-[p-Ser383]-Antibody-Immunoprecipitation-NB100-208-img0001.jpg "Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.")
![Western Blot: COX-2 Antibody (10B2) [NBP3-26361] - Positive Western Blot detected in: U-87 whole cell lysate. All lanes: COX2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69 kDa Observed band size: 69 kDa](http://images.novusbio.com/fullsize/nbp3-26361_rabbit-cox-2-mab-10b2-282202411442620.jpg "Western Blot: COX-2 Antibody (10B2) [NBP3-26361] - Positive Western Blot detected in: U-87 whole cell lysate. All lanes: COX2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69 kDa Observed band size: 69 kDa")
![Immunocytochemistry/Immunofluorescence: COX-2 Antibody (10B2) [NBP3-26361] - Staining of HepG2 Cells with COX-2 Antibody (10B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26361_rabbit-cox-2-mab-10b2-282202412475140.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody (C1) [NBP3-42078] - DAB staining on IHC-P; Sample: Mouse Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-42078; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42078_mouse-ret-mab-c1-612202421331684.jpg "Immunohistochemistry-Paraffin: Ret Antibody (C1) [NBP3-42078] - DAB staining on IHC-P; Sample: Mouse Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-42078; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Ret Antibody (C1) [NBP3-42078] - Sample: K562 cell lysate Primary Ab: 3ug/ml NBP3-42078; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42078_mouse-ret-mab-c1-61220242126298.jpg)
![Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71857-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Girdin Antibody [NBP1-71857] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71856 , which recognizes an upstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71857-img0001.jpg)
![Immunohistochemistry: LRG1 Antibody (7A1) [NBP3-26164] - Image of LRG1 Antibody (7A1) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26164_rabbit-lrg1-mab-7a1-27220248274911.jpg "Immunohistochemistry: LRG1 Antibody (7A1) [NBP3-26164] - Image of LRG1 Antibody (7A1) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: LRG1 Antibody (7A1) [NBP3-26164] - Positive Western Blot detected in: L02 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: LRG1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa Observed band size: 39 kDa](http://images.novusbio.com/fullsize/nbp3-26164_rabbit-lrg1-mab-7a1-282202411351610.jpg)
![Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32072. For blotting immunoprecipitated KAP-1, NBP2-32072 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32072-img0003.jpg)
![Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-Bovine cardiac preparation treated with PKA (lane1) or untreated (lane 2), was probed with anti-phospho-phospholamban (0.5 ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp3-18744_rabbit-phospholamban-p-ser16-pab-western-blot-1992023132527..jpg "Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-Bovine cardiac preparation treated with PKA (lane1) or untreated (lane 2), was probed with anti-phospho-phospholamban (0.5 ug/ml).")
![Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32071-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32071. For blotting immunoprecipitated KAP-1, NBP2-32071 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32071-img0004.jpg)
![Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] - Image of CD44 Antibody (4B7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26681_rabbit-cd44-mab-4b7-27220248203910.jpg "Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] - Image of CD44 Antibody (4B7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: CD44 Antibody (4B7) [NBP3-26681] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: CD44 Antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82 KDa Observed band size: 82 KDa](http://images.novusbio.com/fullsize/nbp3-26681_rabbit-cd44-mab-4b7-2822024114098.jpg)
![Western Blot: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: Glycogen Synthase [p Ser641] Antibody at 1.12ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85 KDa Observed band size: 85 KDa](http://images.novusbio.com/fullsize/nbp3-26421_rabbit-glycogen-synthase-p-ser-641-mab-1d1-28220241148588.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: Glycogen Synthase [p Ser641] Antibody at 1.12ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85 KDa Observed band size: 85 KDa")
![Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (1D1) [NBP3-26421] - Staining of HepG2 cells with Glycogen Synthase [p Ser641] Antibody (1D1) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26421_rabbit-glycogen-synthase-p-ser-641-mab-1d1-282202412493634.jpg)
![Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Image of SHP-2/PTPN11 Antibody (6G11) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26357_rabbit-shp-2-ptpn11-mab-6g11-262202414471577.jpg "Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Image of SHP-2/PTPN11 Antibody (6G11) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Positive Western Blot detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate. All lanes: SHP-2/PTPN11 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 53 kDa Observed band size: 69 kDa](http://images.novusbio.com/fullsize/nbp3-26357_rabbit-shp-2-ptpn11-mab-6g11-282202411472116.jpg)