| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26680-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79851 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33983-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26384-50ul | |||
![Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251734544.jpg "Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.")
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517315598.jpg)
![Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517344236.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody (9W6E7) [CD44] - Confocal imaging of HeLa cells using CD44 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517352722.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and HeLa cells were surface-stained with CD44 Rabbit mAb or ABflo 647 Rabbit IgG isotype control , followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-5220251735270.jpg)
![Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control or CD44 Rabbit mAb .](http://images.novusbio.com/fullsize/nbp3-15617_rabbit-cd44-mab-9w6e7-52202517304969.jpg)
![ELISA: Rat CD44 ELISA Kit (Colorimetric) [NBP2-75154] - Samples were spiked with high concentrations of Rat CD44 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75154_rat-cd44-elisa-kit-colorimetric-132202416201914.jpg "ELISA: Rat CD44 ELISA Kit (Colorimetric) [NBP2-75154] - Samples were spiked with high concentrations of Rat CD44 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CD44 ELISA Kit (Colorimetric) [NBP2-75154] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75154_rat-cd44-elisa-kit-colorimetric-432024856714.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg)
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg)
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-2622024152247.jpg "Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.")
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-262202415253312.jpg)
![Western Blot: CD44 Antibody (19H12) [NBP3-26680] - Positive Western Blot detected in: A549 whole cell lysate, MCF7 whole cell lysate, HepG2 whole cell lysate.<br /> All lanes: CD44 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 81 kDa<br /> Observed band size: 81 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241136303.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (19H12) [NBP3-26680] - Staining of Hela with CD44 Antibody (19H12) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 515-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241323630.jpg)
![Flow Cytometry: CD44 Antibody (19H12) [NBP3-26680] - Overlay Peak curve showing Hela cells stained with CD44 Antibody (19H12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-282202414312756.jpg)
![Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).](http://images.novusbio.com/fullsize/IL-3R-alpha-CD123-Antibody-IL3RA-2947R-Immunohistochemistry-Paraffin-NBP2-79717-img0001.jpg "Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).")
![Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IL-3R alpha/CD123 Antibody (IL3RA/2947R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/IL-3R-alpha-CD123-Antibody-IL3RA-2947R-Protein-Array-NBP2-79717-img0002.jpg)
![Western Blot: EEF1A2 Antibody [NBP2-33983] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP2-33983-img0002.jpg "Western Blot: EEF1A2 Antibody [NBP2-33983] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33983] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-33983-img0001.jpg)
![Western Blot: EEF1A2 Antibody [NBP1-32555] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-32555-img0005.jpg "Western Blot: EEF1A2 Antibody [NBP1-32555] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EEF1A2 Antibody [NBP1-32555] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/EEF1A2-Antibody-Western-Blot-NBP1-32555-img0002.jpg)
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] - EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.EEF1A2 stained by EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32555_rabbit-polyclonal-eef1a2-antibody-910202420115046.jpg)
![Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] - EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.EEF1A2 stained by EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32555_rabbit-polyclonal-eef1a2-antibody-910202420131281.jpg)
![Western Blot: EEF1A2 Antibody [NBP1-32555] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32555_rabbit-polyclonal-eef1a2-antibody-91020242015721.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : <br>A. 30 ug HeLa whole cell lysate/extract (untreated). <br>B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Western-Blot-NBP2-43620-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : <br>A. 30 ug HeLa whole cell lysate/extract (untreated). <br>B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Mouse brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0006.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0005.jpg)
![Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate.<br /> All lanes: PIK3CA Antibody at 1: 1500<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 125 kDa<br /> Observed band size: 110 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202411442616.jpg "Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate.<br /> All lanes: PIK3CA Antibody at 1: 1500<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 125 kDa<br /> Observed band size: 110 kDa<br />")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] - Staining of Hela Cells with PIK3CA Antibody (10E5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202412493610.jpg)