| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26248-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21116 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14018 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95103-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87829-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87405-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-183SS | |||
![Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - analysis of HeLa cell lysate (30ug) using anti-Ku80 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NBP1-56408-img0009.jpg "Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - analysis of HeLa cell lysate (30ug) using anti-Ku80 antibody. Image from verified customer review.")
![Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - Titration: 1.25ug/ml, Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NBP1-56408-img0006.jpg)
![Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - Titration: 1 ug/ml Positive Control: Human pancreatic cancer cell line MiaPaca-2 and Panc-1.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NBP1-56408-img0005.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-30878] - HBL435 xenograft. XRCC4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/XRCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-30878-img0007.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-30878] - HBL435 xenograft. XRCC4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-30878]- HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: XRCC4 stained by XRCC4 antibody diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-30878_rabbit-polyclonal-xrcc4-antibody-immunocytochemistry-immunofluorescence-1912202373956..jpg)
![Western Blot: XRCC4 Antibody [NBP1-30878] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-30878_rabbit-polyclonal-xrcc4-antibody-western-blot-1912202374136..jpg)
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-262202414471526.jpg "Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Image of Ku80/XRCC5 Antibody (5G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-2722024827496.jpg)
![Western Blot: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Positive Western Blot detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: Ku80/XRCC5 Antibody at 1: 2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 83 kDa Observed band size: 83 kDa](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-282202411442624.jpg)
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5G3) [NBP3-26248] - Staining of Hela Cells with Ku80/XRCC5 Antibody (5G3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26248_rabbit-ku80-xrcc5-mab-5g3-28220241312241.jpg)
![Western Blot: Ku80/XRCC5 Antibody (XRCC5/7317) [NBP3-13796] - Western blot analysis of MOLT-4 and HEK293 cell lysates using Ku80/XRCC5 antibody (XRCC5/7317).](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-XRCC5-7317-Western-Blot-NBP3-13796-img0001.jpg "Western Blot: Ku80/XRCC5 Antibody (XRCC5/7317) [NBP3-13796] - Western blot analysis of MOLT-4 and HEK293 cell lysates using Ku80/XRCC5 antibody (XRCC5/7317).")
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (XRCC5/7317) [NBP3-13796] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Ku80/XRCC5 antibody (XRCC5/7317). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-XRCC5-7317-Immunohistochemistry-Paraffin-NBP3-13796-img0004.jpg)
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (XRCC5/7317) [NBP3-13796] - Formalin-fixed, paraffin-embedded human lymph node stained with Ku80/XRCC5 antibody (XRCC5/7317). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-XRCC5-7317-Immunohistochemistry-Paraffin-NBP3-13796-img0002.jpg)
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (XRCC5/7317) [NBP3-13796] - Formalin-fixed, paraffin-embedded human kidney stained with Ku80/XRCC5 antibody (XRCC5/7317). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-XRCC5-7317-Immunohistochemistry-Paraffin-NBP3-13796-img0003.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-659] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-659. DNA-PKcs was also IPed using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-658 and NB100-660.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-659-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-659] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-659. DNA-PKcs was also IPed using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-658 and NB100-660.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NB100-659] - Human lung carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Immunohistochemistry-Paraffin-NB100-659-img0002.jpg)
![Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of K-562 cells, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NBP2-95103-img0004.jpg "Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of K-562 cells, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Mre11 Antibody [NBP2-95103] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Mre11 Rabbit pAb (NBP2-95103) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NBP2-95103-img0003.jpg)
![Immunohistochemistry-Paraffin: Mre11 Antibody [NBP2-95103] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Mre11 Rabbit pAb (NBP2-95103) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NBP2-95103-img0002.jpg)
![Western Blot: Mre11 Antibody [NBP2-95103] - Western blot analysis of extracts of various cell lines, using Mre11 antibody (NBP2-95103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Mre11 Antibody-Western Blot-NBP2-95103-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87829-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-87829] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-87829] - Staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunohistochemistry-Paraffin-NBP1-87829-img0002.jpg)
![Western Blot: Ku80/XRCC5 Antibody [NBP1-87829] -Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/antibody/nbp1-87829_rabbit-polyclonal-ku80-xrcc5-antibody-western-blot-1042025113655..png)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0005.jpg "Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells and smooth muscle.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0004.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP1-87405] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP1-87405-img0006.jpg)
![Western Blot: Artemis Antibody [NB100-183] - Detection of Artemis protein in human testis lysate using NB 100-183.](http://images.novusbio.com/fullsize/Artemis-Antibody-Western-Blot-NB100-183-img0001.jpg "Western Blot: Artemis Antibody [NB100-183] - Detection of Artemis protein in human testis lysate using NB 100-183.")
![Western Blot: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Western blot analysis of DNA-PKcs on different lysates with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lane 6: HT-29 cell lysate Lane 7: A549 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 23 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32272) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-27820241529553.jpg "Western Blot: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Western blot analysis of DNA-PKcs on different lysates with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lane 6: HT-29 cell lysate Lane 7: A549 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 23 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32272) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs antibody (NBP3-32272) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32272) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-298202414333675.jpg)
![Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (2-B3-R) [NBP3-32272] - Immunocytochemistry analysis of HepG2 cells labeling DNA-PKcs with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DNA-PKcs antibody (NBP3-32272) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32272_mouse-dna-pkcs-mab-2-b3-r-29202410334012.jpg)