| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85563-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84412-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50050SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26573-50ul | |||
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Western-Blot-NBP2-45522-img0006.jpg "Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0005.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0001.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0002.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0003.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0004.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517315564.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.")
![Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Confocal imaging of Neuro-2a cells using Enolase 2/Neuron-specific Enolase Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-5220251733389.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361231.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517333854.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361234.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0004.jpg "Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: KCNN3 Antibody [NBP2-85137] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN3. Gene Name: KCNN3. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-NBP2-85137-img0006.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0002.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - KCNN3 antibody - C-terminal region validated by WB using Mouse kidney, Whole brain lysate at 2 ug/ml.](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0003.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human 293T Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNN3 Antibody [NBP2-85137] - Rabbit Anti-KCNN3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Membrane. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3. Secondary Antibody Concentration: 1:200. Magnificatio](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-Paraffin-NBP2-85137-img0001.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Knockout-Validated-NBP1-92688-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [NBP1-92688] - Immunocytochemistry/Immunofluorescence: [NBP1-92688] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Immunocytochemistry-Immunofluorescence-NBP1-92688-img0005.jpg)
![Western Blot: Vimentin Antibody (2A52) [NBP1-92688] - Analysis of cell and whole brain tissue lysates using mouse mAb to vimentin, NBP1-92688, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] C6, and [7] rat brain. The band at about 50kDa mark corresponds to vimentin protein. The antibody does not recognize mouse vimentin (not shown).](http://images.novusbio.com/fullsize/Vimentin-Antibody-2A52-Western-Blot-NBP1-92688-img0006.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP1-85563] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85563-img0010.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody [NBP1-85563] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP1-85563] - Staining of human cerebral cortex shows very strong granular positivity in cytoplasm in neurons.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85563-img0007.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP1-85563] - Staining of human lung shows very strong granular positivity in cytoplasm in macrophages.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85563-img0008.jpg)
![Immunohistochemistry-Paraffin: PPT1 Antibody [NBP1-85563] - Staining of human prostate shows strong granular positivity in cytoplasm in glandular cells.](http://images.novusbio.com/fullsize/PPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85563-img0009.jpg)
![Western Blot: ASPA Antibody [NBP1-89258] - Analysis in control (vector only transfected HEK293T lysate) and ASPA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ASPA-Antibody-Western-Blot-NBP1-89258-img0007.jpg "Western Blot: ASPA Antibody [NBP1-89258] - Analysis in control (vector only transfected HEK293T lysate) and ASPA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Analysis in human kidney and tonsil tissues using NBP1-89258 antibody. Corresponding ASPA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0012.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0008.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0009.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0010.jpg)
![Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-89258] - Staining of human tonsil shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/ASPA-Antibody-Immunohistochemistry-Paraffin-NBP1-89258-img0011.jpg)
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845324.jpg "Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845328.jpg)
![Western Blot: c-Fos Antibody (14C10) [NBP3-26573] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: c-Fos Antibody at 0.81ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 29, 37 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202411351611.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (14C10) [NBP3-26573] - Staining of HepG2 cells with c-Fos Antibody (14C10) at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-28220241249368.jpg)
![Flow Cytometry: c-Fos Antibody (14C10) [NBP3-26573] - Overlay histogram showing Hela cells stained with c-Fos Antibody (14C10) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202414183668.jpg)