| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81060-25ul | |||
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-19920231856919.jpg "Western Blot: eNOS Antibody (M221) [NBP3-23209] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: eNOS Antibody (M221) [NBP3-23209] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23209_mouse-enos-mab-m221-11920231633165.jpg)
![Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-1992023141014.jpg "Immunocytochemistry/Immunofluorescence: eNOS Antibody [NBP3-23210] - Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NBP3-23209) and rabbit polyclonal eNOS (NBP3-23210) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: eNOS Antibody [NBP3-23210] - Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23210_rabbit-enos-pab-19920231856933.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231856948.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: nNOS Antibody (M401) [NBP3-23224] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231820296.jpg)
![Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Western-Blot-NBP2-15159-img0005.jpg "Western Blot: VAMP-1 Antibody [NBP2-15159] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - VAMP-1 antibody detects VAMP-1 protein on mouse fore at Dilution: 1:500.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0008.jpg)
![Immunohistochemistry-Paraffin: VAMP-1 Antibody [NBP2-15159] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15159-img0004.jpg)
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human pancreas shows membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0011.jpg "Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human pancreas shows membranous positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human cerebellum shows positivity in neuropil.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0009.jpg)
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody [NBP1-81060] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/AHNAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81060-img0008.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody [NBP1-81060] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: AHNAK Antibody [NBP1-81060] - Staining of human oral mucosa shows cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/AHNAK-Antibody-Immunohistochemistry-Paraffin-NBP1-81060-img0007.jpg)
![Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Mouse Placenta lysate Primary Ab: 1ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-61220242151879.jpg "Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Mouse Placenta lysate Primary Ab: 1ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Noggin Antibody (A9) [NBP3-41765] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30ug/ml NBP3-41765; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-612202421121379.jpg)
![Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Rat Placenta lysate; Primary Ab: 2ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-612202421395335.jpg)
![Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Western-Blot-NB100-1801-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Gemin 3 Antibody [NB100-1801] - Detection of Human DDX20 on HeLa whole cell lystate using NB100-1801. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibody NB100-1802 and two.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Gemin 3 Antibody [NB100-1801] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX20 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Gemin-3-Antibody-Immunohistochemistry-NB100-1801-img0003.jpg)