| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22397-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41364-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11943-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81038-0.025mg | |||
![Western Blot: Musashi-1 Antibody (9S7Y1) [NBP3-16754] - Western blot analysis of extracts of various cell lines, using Musashi-1 Rabbit mAb (NBP3-16754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-9S7Y1-Western-Blot-NBP3-16754-img0003.jpg "Western Blot: Musashi-1 Antibody (9S7Y1) [NBP3-16754] - Western blot analysis of extracts of various cell lines, using Musashi-1 Rabbit mAb (NBP3-16754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (9S7Y1) [NBP3-16754] - Immunofluorescence analysis of U-2 OS cells using Musashi-1 Rabbit mAb (NBP3-16754) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-9S7Y1-Immunocytochemistry-Immunofluorescence-NBP3-16754-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (9S7Y1) [NBP3-16754] - Immunofluorescence analysis of NIH-3T3 cells using Musashi-1 Rabbit mAb (NBP3-16754) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-9S7Y1-Immunocytochemistry-Immunofluorescence-NBP3-16754-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (9S7Y1) [NBP3-16754] - Immunofluorescence analysis of U-2 OS cells using Musashi-1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16754_rabbit-musashi-1-mab-9s7y1-52202511361286.jpg%3Ft=1698886538)
![Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-91220241418136.jpg "Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Mouse cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-91220241581233.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Confocal imaging of paraffin-embedded Rat eyeball tissue using Cytokeratin 12 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-5220251255535.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Confocal imaging of paraffin-embedded Mouse eyeball tissue using Cytokeratin 12 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-33197_rabbit-cytokeratin-12-mab-2m1k10-5220251215484.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NB300-176-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.")
![Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Western-Blot-NBP2-22397-img0003.jpg "Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-2C-Adrenergic-R-ADRA2C-Antibody-S330A-80-Immunocytochemistry-Immunofluorescence-NBP2-22397-img0005.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Immunohistochemistry-NBP2-41364-img0005.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]")
![Sandwich ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364] - Analysis using the Biotin conjugate of NBP2-41364. The calibration curveusing NBP2-23595 as the biotinylated detection antibody and NBP2-41364 as the capture antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-14-BSA-Free-Sandwich-ELISA-NBP2-41364-img0004.jpg)
![Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-278202416285228.jpg "Western Blot: CPT1A Antibody (PSH04-00) [NBP3-32213] - Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32213) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (NBP3-32213) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32213) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29820241435278.jpg)
![Flow Cytometry: CPT1A Antibody (PSH04-00) [NBP3-32213] - Flow cytometric analysis of HepG2 cells labeling CPT1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32213, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32213_rabbit-cpt1a-mab-psh04-00-29202416453278.jpg)
![Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/IL-6-Antibody-1-6-Western-Blot-NBP2-89149-img0001.jpg "Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of EL4 cells labeling IL-6 with Rabbit anti-IL-6 antibody at 1/1,000 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-6 antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-89149_mouse-monoclonal-il-6-antibody-1-6-immunocytochemistry-immunofluorescence-266202412753..jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)
![Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Western-Blot-NBP2-81078-img0002.jpg "Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.")
![Immunocytochemistry /Immunofluorescence: PrPSc Antibody (3F4) - Azide and BSA Free [NBP2-81078] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81078 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81078, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81078_mouse-monoclonal-prpsc-antibody-3f4-74202522265212.png)
![Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Western-Blot-NBP3-11943-img0001.jpg "Western Blot: A2BP1 Antibody (1G10) [NBP3-11943] - Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa A2BP1 protein.")
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (1G10) [NBP3-11943] - Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of A2BP1 (cat. NBP3-11943, 1:500, green).](http://images.novusbio.com/fullsize/A2BP1-Antibody-1G10-Immunocytochemistry-Immunofluorescence-NBP3-11943-img0002.jpg)
![Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-83-14-Flow-Cytometry-NBP2-81038-img0001.jpg "Flow Cytometry: Insulin R/CD220 Antibody (83-14) [NBP2-81038] - Flow-cytometry using the Insulin R/CD220 Antibody (83-14) [NBP2-81038]. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (NBP2-81038, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54355-img0001.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/1806R)")
![Flow Cytometry: CD34 Antibody (HPCA1/1806R) - Azide and BSA Free [NBP2-54355] - Flow Cytometric Analysis of human Jurkat cells using CD34 Antibody (HPCA1/1806R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1806R-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54355-img0002.jpg)