| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22410-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37276-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89836-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
![Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Western-Blot-NBP2-22410-img0002.jpg "Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.")
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGCNL1Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: VGCNL1 Antibody (S187-7) [NBP2-22410] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGCNL1Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGCNL1-Antibody-S187-7-Immunocytochemistry-Immunofluorescence-NBP2-22410-img0004.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0003.jpg)
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-ELISA-NBP2-37276-img0001.jpg)
![Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-70558-img0001.jpg "Western Blot: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecor")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70558-img0003.jpg)
![Flow Cytometry: Doublecortin Antibody (OTI4A3) - Azide and BSA Free [NBP2-70558] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-OTI4A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70558-img0002.jpg)
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0003.jpg "Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69992-img0002.jpg "Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-6ug-ml-staining-of-paraffin-embedded-Human-Cortex-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254027.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-Negative-Control-showing-staining-of-paraffin-embedded-Human-Cortex-with-no-primary-antibody--29320239254010.jpg)
![Flow Cytometry: Doublecortin Antibody [NBP2-69992] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Flow-Cytometry-NBP2-69992-img0001.jpg)
![Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg)
![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89836_rabbit-monoclonal-crabp2-antibody-004-235202514331567.png)
![Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-278202415295538.jpg "Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Kir2.1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32505, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-298202414333623.jpg)
![Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505] - ICC staining of Kir2.1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32505, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-29202410484869.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of retina](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-NBP3-14401-img0005.jpg "Immunohistochemistry: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of retina")
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of formalin-fixed, paraffin-embedded human retina after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NBP3-14401-img0001.jpg)
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NBP3-14401-img0002.jpg)
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NBP3-14401-img0003.jpg)
![Immunohistochemistry-Paraffin: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of formalin-fixed, paraffin-embedded human eye after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Melatonin-Receptor-1B-Antibody-Immunohistochemistry-Paraffin-NBP3-14401-img0004.jpg)
![Immunohistochemistry: Melatonin Receptor 1B Antibody [NBP3-14401] - Immunohistochemistry of brain, glioblastoma,](http://images.novusbio.com/fullsize/Melatonin Receptor 1B Antibody-Immunohistochemistry-NBP3-14401-img0006.jpg)
![Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-288202412461639.jpg "Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PON1 Antibody (JE30-90) [NBP3-32843] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-PON1 antibody (NBP3-32843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-298202414304136.jpg)
![Flow Cytometry: PON1 Antibody (JE30-90) [NBP3-32843] - Flow cytometric analysis of HepG2 cells labeling PON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32843, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-29202416453293.jpg)
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Western-Blot-NBP2-67207-img0001.jpg "Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0004.jpg)
![Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Flow cytometric analysis of CaM Kinase II alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Flow-Cytometry-NBP2-67207-img0002.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaM Kinase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0003.jpg)
![Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Western-Blot-NBP1-42461-img0002.jpg "Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.")
![SDS-Page: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - This product has a purity >95%. This protein was run on a 4-20% SDS-PAGE gel with 5 ug protein loaded.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Immunoblotting-NBP1-42461-img0001.jpg)